دورية أكاديمية
Superfast Gelation of Spider Silk-Based Artificial Silk Protein
| العنوان: | Superfast Gelation of Spider Silk-Based Artificial Silk Protein |
|---|---|
| المؤلفون: | Fan Wen, Yu Wang, Bowen Tu, Lun Cui |
| المصدر: | Gels, Vol 10, Iss 1, p 69 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Science LCC:Chemistry LCC:Inorganic chemistry LCC:General. Including alchemy |
| مصطلحات موضوعية: | spider silk proteins, rapid gelation, hydrogel formation, self-assembly, recombinant protein, Science, Chemistry, QD1-999, Inorganic chemistry, QD146-197, General. Including alchemy, QD1-65 |
| الوصف: | Spider silk proteins (spidroins) have garnered attention in biomaterials research due to their ability to self-assemble into hydrogels. However, reported spidroin hydrogels require high protein concentration and prolonged gelation time. Our study engineered an artificial spidroin that exhibits unprecedented rapid self-assembly into hydrogels at physiologically relevant conditions, achieving gelation at a low concentration of 6 mg/mL at 37 °C without external additives. Remarkably, at a 30 mg/mL concentration, our engineered protein forms hydrogels within 30 s, a feature we termed “superfast gelation”. This rapid formation is modulated by ions, pH, and temperature, offering versatility in biomedical applications. The hydrogel’s capacity to encapsulate proteins and support E. coli growth while inducing RFP expression provides a novel platform for drug delivery and bioengineering applications. Our findings introduce a superfast, highly adaptable, and cytocompatible hydrogel that self-assembles under mild conditions, underscoring the practical implication of rapid gelation in biomedical research and clinical applications. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2310-2861 |
| Relation: | https://www.mdpi.com/2310-2861/10/1/69; https://doaj.org/toc/2310-2861 |
| DOI: | 10.3390/gels10010069 |
| URL الوصول: | https://doaj.org/article/1c2566a5ee6d45ecab36aec5ede72a2c |
| رقم الأكسشن: | edsdoj.1c2566a5ee6d45ecab36aec5ede72a2c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23102861 |
|---|---|
| DOI: | 10.3390/gels10010069 |