دورية أكاديمية
Visualization of Leishmania tropica Infection in BALB/c Mice by Bioluminescence Imaging
| العنوان: | Visualization of Leishmania tropica Infection in BALB/c Mice by Bioluminescence Imaging |
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| المؤلفون: | Mahdieh Eskandar, Elham Gholami, Negar Seyed, Yasaman Taslimi, Sima Habibzadeh, Sima Rafati, Tahereh Taheri |
| المصدر: | Iranian Biomedical Journal, Vol 24, Iss 3, Pp 164-172 (2020) |
| بيانات النشر: | Pasteur Institute of Iran, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | balb/c mice, green fluorescent protein, leishmania tropica, luciferase, Medicine |
| الوصف: | Background: Leishmania tropica is the cause of more than one form of leishmaniasis and lacks a known reservoir animal. This study compares the potential infectivity of recombinant and wild-type L. tropica in BALB/c mice. Methods: The potential infectivity of recombinant L. tropicaEGFP or L. tropicaEGFP-LUC by two different, the subcutaneous and intradermal, routes was compared using a range of classical detection methods and bioluminescence imaging (BLI). Results: In addition to the results obtained from classical diagnostic approaches, the BLI signals were detected in footpads and ears of L. tropica-infected animals. The BLI revealed that a bioluminescence signal can be observed at the inoculation site. The stability of the BLI remained constant in the footpad, but the signal was detectable for only three months in the pinna due to the decline in infection over time. Conclusion: The presented data are a precise verification of the assumption that BALB/c mice could be used as an experimental model for L. tropica infectivity. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1028-852X 2008-823X |
| Relation: | http://ibj.pasteur.ac.ir/article-1-2969-en.html; https://doaj.org/toc/1028-852X; https://doaj.org/toc/2008-823X |
| URL الوصول: | https://doaj.org/article/1c30ef5a9a7447b784cb7e579f39bec9 |
| رقم الأكسشن: | edsdoj.1c30ef5a9a7447b784cb7e579f39bec9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1028852X 2008823X |
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