دورية أكاديمية
De novo protein structure determination by heavy-atom soaking in lipidic cubic phase and SIRAS phasing using serial synchrotron crystallography
| العنوان: | De novo protein structure determination by heavy-atom soaking in lipidic cubic phase and SIRAS phasing using serial synchrotron crystallography |
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| المؤلفون: | S. Botha, D. Baitan, K. E. J. Jungnickel, D. Oberthür, C. Schmidt, S. Stern, M. O. Wiedorn, M. Perbandt, H. N. Chapman, C. Betzel |
| المصدر: | IUCrJ, Vol 5, Iss 5, Pp 524-530 (2018) |
| بيانات النشر: | International Union of Crystallography, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Crystallography |
| مصطلحات موضوعية: | serial crystallography, SIRAS phasing, lipidic cubic phase, heavy-atom soaking, de novo protein structure determination, Crystallography, QD901-999 |
| الوصف: | During the past few years, serial crystallography methods have undergone continuous development and serial data collection has become well established at high-intensity synchrotron-radiation beamlines and XFEL radiation sources. However, the application of experimental phasing to serial crystallography data has remained a challenging task owing to the inherent inaccuracy of the diffraction data. Here, a particularly gentle method for incorporating heavy atoms into micrometre-sized crystals utilizing lipidic cubic phase (LCP) as a carrier medium is reported. Soaking in LCP prior to data collection offers a new, efficient and gentle approach for preparing heavy-atom-derivative crystals directly before diffraction data collection using serial crystallography methods. This approach supports effective phasing by utilizing a reasonably low number of diffraction patterns. Using synchrotron radiation and exploiting the anomalous scattering signal of mercury for single isomorphous replacement with anomalous scattering (SIRAS) phasing resulted in high-quality electron-density maps that were sufficient for building a complete structural model of proteinase K at 1.9 Å resolution using automatic model-building tools. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2052-2525 20522525 |
| Relation: | http://scripts.iucr.org/cgi-bin/paper?S2052252518009223; https://doaj.org/toc/2052-2525 |
| DOI: | 10.1107/S2052252518009223 |
| URL الوصول: | https://doaj.org/article/1c3d5c2798924d649b56a82d7d3cbf26 |
| رقم الأكسشن: | edsdoj.1c3d5c2798924d649b56a82d7d3cbf26 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20522525 |
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| DOI: | 10.1107/S2052252518009223 |