دورية أكاديمية
Extremophile enzyme activity lab: using catalase from Pyrobaculum calidifontis to highlight temperature sensitivity and thermostable enzyme activity
| العنوان: | Extremophile enzyme activity lab: using catalase from Pyrobaculum calidifontis to highlight temperature sensitivity and thermostable enzyme activity |
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| المؤلفون: | Joseph W. Scott, J. Jordan Steel |
| المصدر: | Journal of Microbiology & Biology Education, Vol 25, Iss 1 (2024) |
| بيانات النشر: | American Society for Microbiology, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Special aspects of education LCC:Biology (General) |
| مصطلحات موضوعية: | extremophile, hyperthermophile, catalase enzyme, active learning, lab exercise, Special aspects of education, LC8-6691, Biology (General), QH301-705.5 |
| الوصف: | ABSTRACTThere are places on earth that are considered to possess extreme physico-chemical characteristics as they relate to life. Surprisingly, there are microbes that have adapted various strategies that enable them to form robust communities in these environments. The microbes that live in these environments, called extremophiles, are described as being thermophilic, psychrophilic, halophilic, acidophilic, alkaliphilic, barophilic, and so on. Given that extremophiles were not discovered until relatively recently due to a view point that the environments in which they inhabited were not conducive to life, it is reasonable to conclude that the concept of extremophiles may be hard to grasp for students. Herein is described a laboratory exercise adapted from laboratory exercises that use mesophilic catalase enzymes to illustrate the influence of physico-chemical parameters on enzyme activity. Catalase is an enzyme that accelerates the degradation of hydrogen peroxide to water and oxygen gas. In addition to mesophilic catalases, the catalase from Pyrobaculum calidifontis, a hyperthermophile with an optimal growth temperature of 90°C, is used to highlight the adaptation of an enzyme to an extreme environment. A visual comparison of bubble production by the hyperthermophilic and mesophilic enzymes after heating at high temperatures dramatically illustrates differences in thermostability that will likely reinforce concepts that are given in a pre-laboratory lecture that discusses not only the extremophiles themselves but also their applications in biotechnology and possible role in the field of astrobiology. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1935-7885 1935-7877 |
| Relation: | https://doaj.org/toc/1935-7877; https://doaj.org/toc/1935-7885 |
| DOI: | 10.1128/jmbe.00065-23 |
| URL الوصول: | https://doaj.org/article/d1d30d1dc64e456ebb285403f35fbf53 |
| رقم الأكسشن: | edsdoj.1d30d1dc64e456ebb285403f35fbf53 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19357885 19357877 |
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| DOI: | 10.1128/jmbe.00065-23 |