دورية أكاديمية
PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells
| العنوان: | PEGylated gold nanoparticles-ribonuclease induced oxidative stress and apoptosis in colorectal cancer cells |
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| المؤلفون: | Mostafa Akbarzadeh Khiavi, Azam Safary, Jaleh Barar, Hamed Farzi-Khajeh, Abolfazl Barzegari, Rahimeh Mousavi, Mohammad Hossein Somi, Yadollah Omidi |
| المصدر: | BioImpacts, Vol 10, Iss 1, Pp 27-36 (2020) |
| بيانات النشر: | Tabriz University of Medical Sciences, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | bovine pancreatic ribonuclease, colorectal cancer, gold nanoparticles, nanomedicine, pegylation, reactive oxygen species, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | Introduction: Currently, drug-induced reactive oxygen species (ROS) mediating apoptosis pathway have extensively been investigated in designing effective strategies for colorectal cancer (CRC) chemotherapy. Bovine pancreatic ribonuclease A (RNase A) represents a new class of cytotoxic and non-mutagenic enzymes, and has gained more attention as a potential anticancer modality; however, the cytosolic ribonuclease inhibitors (RIs) restrict the clinical application of this enzyme. Nowadays, nanotechnology-based diagnostic and therapeutic systems have provided potential solutions for cancer treatments. Methods: In this study, the gold nanoparticles (AuNPs) were synthesized, stabilized by polyethylene glycol (PEG), functionalized, and covalently conjugated with RNase A. The physicochemical properties of engineered nanobiomedicine (AuNPs-PEG-RNase A) were characterized by scanning electron microscope (SEM), dynamic light scattering (DLS), and UV-vis spectrum. Then, its biological impacts including cell viability, apoptosis, and ROS production were evaluated in the SW-480 cells. Results: The engineered nanobiomedicine, AuNPs-PEG-RNase A, was found to effectively induce apoptosis in SW-480 cells and result in a significant reduction in cancer cell viability. Besides, the maximum production of ROS was obtained after the treatment of cells with an IC50 dose of AuNPs-PEG-RNase A. Conclusion: Based on the efficient ROS-responsiveness and the anticancer activity of RNase A of the engineered nanomedicine, this nanoscaled biologics may be considered as a potential candidate for the treatment of CRC. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2228-5660 2228-5652 |
| Relation: | https://bi.tbzmed.ac.ir/PDF/bi-10-27.pdf; https://doaj.org/toc/2228-5660; https://doaj.org/toc/2228-5652 |
| DOI: | 10.15171/bi.2020.04 |
| URL الوصول: | https://doaj.org/article/1d773cc4fb35482baa55337886a2c04e |
| رقم الأكسشن: | edsdoj.1d773cc4fb35482baa55337886a2c04e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22285660 22285652 |
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| DOI: | 10.15171/bi.2020.04 |