دورية أكاديمية
Changes in proteolytic bacteria in paddy soils in response to organic management
| العنوان: | Changes in proteolytic bacteria in paddy soils in response to organic management |
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| المؤلفون: | Shu Wang, Xiping Cheng |
| المصدر: | Acta Agriculturae Scandinavica. Section B, Soil and Plant Science, Vol 67, Iss 7, Pp 583-589 (2017) |
| بيانات النشر: | Taylor & Francis Group, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Plant culture |
| مصطلحات موضوعية: | organic management, rice, proteolytic bacterial communities, dgge, qpcr, Plant culture, SB1-1110 |
| الوصف: | Proteolytic bacterial communities, which mineralize organic nitrogen, play a key role in agricultural systems. In this study, alkaline metalloprotease (apr) gene fragments from proteolytic bacteria were investigated in bulk and rhizosphere paddy soil from four fields under organic management (for 2, 3, 5, and 9 years), and from one field under conventional management (for 2 years). We analyzed the abundance and structure of the proteolytic bacterial communities using real-time quantitative PCR and denaturing gradient gel electrophoresis. Our results showed that the abundance of proteolytic bacteria ranged from 1.57 × 108 to 8.02 × 108 copies/g of soil. In addition, the abundance of the proteolytic bacteria in the paddy soils under organic management was significantly higher than those in the paddy soil under conventional management. Moreover, the gene copy numbers in the rhizosphere soils were significantly higher than those in the bulk soils. The abundance of proteolytic bacteria tended to increase with the duration of organic management, with the highest abundance being found in the soil that had been under organic management for 5 years. However, the proteolytic bacteria communities in the paddy soils were not significantly affected by management practices. Phylogeny analysis showed that all gel bands obtained represented genes from Pseudomonas. Additionally, correlation analysis and canonical correspondence analysis showed that C/N, C, and N were important factors that influenced the abundance and community structure of the proteolytic bacteria. These results suggest that proteolytic bacteria are indicators in organic management systems, depolymerize organic N and hence maintain soil sustainability. Abbreviations: CM: conventional management; OM: organic management; DGGE: denaturing gradient gel electrophoresis; qPCR: real-time quantitative PCR detecting system; COFCC: China organic food certification center; CCA: canonical correspondence analysis |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0906-4710 1651-1913 09064710 |
| Relation: | https://doaj.org/toc/0906-4710; https://doaj.org/toc/1651-1913 |
| DOI: | 10.1080/09064710.2017.1320422 |
| URL الوصول: | https://doaj.org/article/ac1daa3eed624371a39617821858cc84 |
| رقم الأكسشن: | edsdoj.1daa3eed624371a39617821858cc84 |
| قاعدة البيانات: | Directory of Open Access Journals |
PDF full text from Publisher | تدمد: | 09064710 16511913 |
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| DOI: | 10.1080/09064710.2017.1320422 |