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Characterization of influenza vaccine immunogenicity using influenza antigen microarrays.

التفاصيل البيبلوغرافية
العنوان: Characterization of influenza vaccine immunogenicity using influenza antigen microarrays.
المؤلفون: Jordan V Price, Justin A Jarrell, David Furman, Nicole H Kattah, Evan Newell, Cornelia L Dekker, Mark M Davis, Paul J Utz
المصدر: PLoS ONE, Vol 8, Iss 5, p e64555 (2013)
بيانات النشر: Public Library of Science (PLoS), 2013.
سنة النشر: 2013
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Existing methods to measure influenza vaccine immunogenicity prohibit detailed analysis of epitope determinants recognized by immunoglobulins. The development of highly multiplex proteomics platforms capable of capturing a high level of antibody binding information will enable researchers and clinicians to generate rapid and meaningful readouts of influenza-specific antibody reactivity.We developed influenza hemagglutinin (HA) whole-protein and peptide microarrays and validated that the arrays allow detection of specific antibody reactivity across a broad dynamic range using commercially available antibodies targeted to linear and conformational HA epitopes. We derived serum from blood draws taken from 76 young and elderly subjects immediately before and 28±7 days post-vaccination with the 2008/2009 trivalent influenza vaccine and determined the antibody reactivity of these sera to influenza array antigens.Using linear regression and correcting for multiple hypothesis testing by the Benjamini and Hochberg method of permutations over 1000 resamplings, we identified antibody reactivity to influenza whole-protein and peptide array features that correlated significantly with age, H1N1, and B-strain post-vaccine titer as assessed through a standard microneutralization assay (p
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
50755145
Relation: http://europepmc.org/articles/PMC3667171?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0064555
URL الوصول: https://doaj.org/article/1e0cb74ce7344a959f7407bf50755145
رقم الأكسشن: edsdoj.1e0cb74ce7344a959f7407bf50755145
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
50755145
DOI:10.1371/journal.pone.0064555