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أعرض في EDS

SUMO1 modification of IGF-1R combining with SNAI2 inhibited osteogenic differentiation of PDLSCs stimulated by high glucose

التفاصيل البيبلوغرافية
العنوان: SUMO1 modification of IGF-1R combining with SNAI2 inhibited osteogenic differentiation of PDLSCs stimulated by high glucose
المؤلفون: Rongrong Jiang, Miao Wang, Xiaobo Shen, Shuai Huang, Jianpeng Han, Lei Li, Zhiliang Xu, Chengfeng Jiang, Qiao Zhou, Xingmei Feng
المصدر: Stem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-14 (2021)
بيانات النشر: BMC, 2021.
سنة النشر: 2021
المجموعة: LCC:Medicine (General)
LCC:Biochemistry
مصطلحات موضوعية: IGF-1R, Differentiation, Periodontal ligament stem cells, SNAI2, High glucose, Medicine (General), R5-920, Biochemistry, QD415-436
الوصف: Abstract Background Periodontal disease, an oral disease characterized by loss of alveolar bone and progressive teeth loss, is the sixth major complication of diabetes. It is spreading worldwide as it is difficult to be cured. The insulin-like growth factor 1 receptor (IGF-1R) plays an important role in regulating functional impairment associated with diabetes. However, it is unclear whether IGF-1R expression in periodontal tissue is related to alveolar bone destruction in diabetic patients. SUMO modification has been reported in various diseases and is associated with an increasing number of biological processes, but previous studies have not focused on diabetic periodontitis. This study aimed to explore the role of IGF-1R in osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in high glucose and control the multiple downstream damage signal factors. Methods PDLSCs were isolated and cultured after extraction of impacted teeth from healthy donors or subtractive orthodontic extraction in adolescents. PDLSCs were cultured in the osteogenic medium with different glucose concentrations prepared by medical 5% sterile glucose solution. The effects of different glucose concentrations on the osteogenic differentiation ability of PDLSCs were studied at the genetic and cellular levels by staining assay, Western Blot, RT-PCR, Co-IP and cytofluorescence. Results We found that SNAI2, RUNX2 expression decreased in PDLSCs cultured in high glucose osteogenic medium compared with that in normal glucose osteogenic medium, which were osteogenesis-related marker. In addition, the IGF-1R expression, sumoylation of IGF-1R and osteogenic differentiation in PDLSCs cultured in high glucose osteogenic medium were not consistent with those cultured in normal glucose osteogenic medium. However, osteogenic differentiation of PDLCSs enhanced after adding IGF-1R inhibitors to high glucose osteogenic medium. Conclusion Our data demonstrated that SUMO1 modification of IGF-1R inhibited osteogenic differentiation of PDLSCs by binding to SNAI2 in high glucose environment, a key factor leading to alveolar bone loss in diabetic patients. Thus we could maximize the control of multiple downstream damage signaling factors and bring new hope for alveolar bone regeneration in diabetic patients.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1757-6512
Relation: https://doaj.org/toc/1757-6512
DOI: 10.1186/s13287-021-02618-w
URL الوصول: https://doaj.org/article/1e124ad57b9549b4b025366d32e4768a
رقم الأكسشن: edsdoj.1e124ad57b9549b4b025366d32e4768a
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:17576512
DOI:10.1186/s13287-021-02618-w