دورية أكاديمية
Estimation of the relative sensitivity of qPCR analysis using pooled samples.
| العنوان: | Estimation of the relative sensitivity of qPCR analysis using pooled samples. |
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| المؤلفون: | Ana Muniesa, Chelo Ferreira, Héctor Fuertes, Nabil Halaihel, Ignacio de Blas |
| المصدر: | PLoS ONE, Vol 9, Iss 4, p e93491 (2014) |
| بيانات النشر: | Public Library of Science (PLoS), 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | The high sensitivity of qPCR makes it a desirable diagnostic method in epidemiological surveillance programs. However, due to high costs, the use of pooling has been suggested. In this paper, an algorithm based on the Montecarlo method has been designed and implemented. The algorithm had been tested in many different situations, and finally it was validated with a real dataset. Moreover, based on the results obtained and depending on pooling conditions, a drastic decrease of sensitivity is observed. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC3983103?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0093491 |
| URL الوصول: | https://doaj.org/article/1ea9d8ebbac94b9fa00c397c9e6927be |
| رقم الأكسشن: | edsdoj.1ea9d8ebbac94b9fa00c397c9e6927be |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0093491 |