دورية أكاديمية

SARS-CoV-2 Accessory Protein ORF8 Targets the Dimeric IgA Receptor pIgR

التفاصيل البيبلوغرافية
العنوان: SARS-CoV-2 Accessory Protein ORF8 Targets the Dimeric IgA Receptor pIgR
المؤلفون: Frederique Laprise, Ariana Arduini, Mathew Duguay, Qinghua Pan, Chen Liang
المصدر: Viruses, Vol 16, Iss 7, p 1008 (2024)
بيانات النشر: MDPI AG, 2024.
سنة النشر: 2024
المجموعة: LCC:Microbiology
مصطلحات موضوعية: SARS-CoV-2, ORF8, pIgR, IgA, IgM, mucosal immunity, Microbiology, QR1-502
الوصف: SARS-CoV-2 is a highly pathogenic respiratory virus that successfully initiates and establishes its infection at the respiratory mucosa. However, little is known about how SARS-CoV-2 antagonizes the host’s mucosal immunity. Recent findings have shown a marked reduction in the expression of the polymeric Ig receptor (pIgR) in COVID-19 patients. This receptor maintains mucosal homeostasis by transporting the dimeric IgA (dIgA) and pentameric IgM (pIgM) across mucosal epithelial cells to neutralize the invading respiratory pathogens. By studying the interaction between pIgR and SARS-CoV-2 proteins, we discovered that the viral accessory protein Open Reading Frame 8 (ORF8) potently downregulates pIgR expression and that this downregulation activity of ORF8 correlates with its ability to interact with pIgR. Importantly, the ORF8-mediated downregulation of pIgR diminishes the binding of dIgA or pIgM, and the ORF8 proteins of the variants of concern of SARS-CoV-2 preserve the function of downregulating pIgR, indicating the importance of this conserved activity of ORF8 in SARS-CoV-2 pathogenesis. We further observed that the secreted ORF8 binds to cell surface pIgR, but that this interaction does not trigger the cellular internalization of ORF8, which requires the binding of dIgA to pIgR. These findings suggest the role of ORF8 in SARS-CoV-2 mucosal immune evasion.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1999-4915
Relation: https://www.mdpi.com/1999-4915/16/7/1008; https://doaj.org/toc/1999-4915
DOI: 10.3390/v16071008
URL الوصول: https://doaj.org/article/c1eb45185c5743d3b47b4e87f9f3534e
رقم الأكسشن: edsdoj.1eb45185c5743d3b47b4e87f9f3534e
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19994915
DOI:10.3390/v16071008