دورية أكاديمية
أعرض في EDS

Gap junctional communication in osteocytes is amplified by low intensity vibrations in vitro.

التفاصيل البيبلوغرافية
العنوان: Gap junctional communication in osteocytes is amplified by low intensity vibrations in vitro.
المؤلفون: Gunes Uzer, Suphannee Pongkitwitoon, Cheng Ian, William R Thompson, Janet Rubin, Meilin E Chan, Stefan Judex
المصدر: PLoS ONE, Vol 9, Iss 3, p e90840 (2014)
بيانات النشر: Public Library of Science (PLoS), 2014.
سنة النشر: 2014
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: The physical mechanism by which cells sense high-frequency mechanical signals of small magnitude is unknown. During exposure to vibrations, cell populations within a bone are subjected not only to acceleratory motions but also to fluid shear as a result of fluid-cell interactions. We explored displacements of the cell nucleus during exposure to vibrations with a finite element (FE) model and tested in vitro whether vibrations can affect osteocyte communication independent of fluid shear. Osteocyte like MLO-Y4 cells were subjected to vibrations at acceleration magnitudes of 0.15 g and 1 g and frequencies of 30 Hz and 100 Hz. Gap junctional intracellular communication (GJIC) in response to these four individual vibration regimes was investigated. The FE model demonstrated that vibration induced dynamic accelerations caused larger relative nuclear displacement than fluid shear. Across the four regimes, vibrations significantly increased GJIC between osteocytes by 25%. Enhanced GJIC was independent of vibration induced fluid shear; there were no differences in GJIC between the four different vibration regimes even though differences in fluid shear generated by the four regimes varied 23-fold. Vibration induced increases in GJIC were not associated with altered connexin 43 (Cx43) mRNA or protein levels, but were dependent on Akt activation. Combined, the in silico and in vitro experiments suggest that externally applied vibrations caused nuclear motions and that large differences in fluid shear did not influence nuclear motion (
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3948700?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0090840
URL الوصول: https://doaj.org/article/1eebac8aaf24406e8192d05065bb2f1b
رقم الأكسشن: edsdoj.1eebac8aaf24406e8192d05065bb2f1b
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0090840