دورية أكاديمية
Spheres derived from lung adenocarcinoma pleural effusions: molecular characterization and tumor engraftment.
| العنوان: | Spheres derived from lung adenocarcinoma pleural effusions: molecular characterization and tumor engraftment. |
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| المؤلفون: | Rita Mancini, Enrico Giarnieri, Claudia De Vitis, Donatella Malanga, Giuseppe Roscilli, Alessia Noto, Emanuele Marra, Carmelo Laudanna, Pietro Zoppoli, Pasquale De Luca, Andrea Affuso, Luigi Ruco, Arianna Di Napoli, Giuseppe Mesiti, Luigi Aurisicchio, Alberto Ricci, Salvatore Mariotta, Lara Pisani, Claudio Andreetti, Giuseppe Viglietto, Erino A Rendina, Maria Rosaria Giovagnoli, Gennaro Ciliberto |
| المصدر: | PLoS ONE, Vol 6, Iss 7, p e21320 (2011) |
| بيانات النشر: | Public Library of Science (PLoS), 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Malignant pleural effusions (MPEs) could represent an excellent source to culture a wide variety of cancer cells from different donors. In this study, we set up culture conditions for cancer cells deriving from MPEs of several patients affected by the most frequent form of lung cancer, namely the subset of non small cell lung cancers (NSCLC) classified as Lung Adenocarcinomas (AdenoCa) which account for approximately 40% of lung cancer cases. AdenoCa malignant pleural effusions gave rise to in vitro cultures both in adherent and/or in spheroid conditions in almost all cases analyzed. We characterized in greater detail two samples which showed the most efficient propagation in vitro. In these samples we also compared gene profiles of spheroid vs adherent cultures and identified a set of differentially expressed genes. Finally we achieved efficient tumor engraftment in recipient NOD/SCID mice, also upon inoculation of small number of cells, thus suggesting indirectly the presence of tumor initiating cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC3138755?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0021320 |
| URL الوصول: | https://doaj.org/article/1f3f48ae59f543fb8128831ee8b65142 |
| رقم الأكسشن: | edsdoj.1f3f48ae59f543fb8128831ee8b65142 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0021320 |