دورية أكاديمية
Characteristics and neural-like differentiation of mesenchymal stem cells derived from foetal porcine bone marrow
| العنوان: | Characteristics and neural-like differentiation of mesenchymal stem cells derived from foetal porcine bone marrow |
|---|---|
| المؤلفون: | Ying Liu, Limei Liu, Xin Ma, Yupeng Yin, Bo Tang, Ziyi Li |
| المصدر: | Bioscience Reports, Vol 33, Iss 2, p e00032 (2013) |
| بيانات النشر: | Portland Press, Biochemical Society, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Life LCC:Microbiology |
| مصطلحات موضوعية: | differentiation, mesenchymal stem cell, octamer-binding transcription factor 4, porcine bone marrow, reverse transcription–PCR, Life, QH501-531, Microbiology, QR1-502 |
| الوصف: | MSCs (mesenchymal stem cells) are a stem cell source that can be easily obtained from bone marrow. Despite the increasing importance of the pig as a large animal model, little is known about foetal pMSCs (porcine MSCs). In this study, we observed the gene expression of pluripotent markers in foetal pMSCs and the capacity of pMSCs to differentiate into adipocytes, osteocytes and neural-like cells using quantitative RT–PCR (reverse transcription–PCR), normal histological staining and immunohistochemistry. Foetal pMSCs have either a spindle or a flattened shape, and flow cytometry revealed the expression of the MSC-related proteins CD44 and CD105 (endoglin) but not CD34 and CD45. pMSCs express pluripotent markers such as Oct4 (octamer-binding transcription factor 4) and Nanog at the protein and mRNA levels. qRT-PCR (quantitative real-time PCR) analyses revealed that pMSCs expressed nestin [for NSCs (neural stem cells)]. Immunocytochemical and RT–PCR data showed that 29% and 23% of pMSCs expressed MAP2 (microtubule-associated protein 2) for neurons and β-tubulin III (Tuj1) for immature neurons, respectively, after induction of neural differentiation. These findings demonstrate the plasticity of pMSCs and their potential for use in cellular replacement therapy for neural diseases. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0144-8463 1573-4935 |
| Relation: | http://www.bioscirep.org/bsr/033/e032/bsr033e032.htm; https://doaj.org/toc/0144-8463; https://doaj.org/toc/1573-4935 |
| DOI: | 10.1042/BSR20120023 |
| URL الوصول: | https://doaj.org/article/a1ffbe2a79cb4a4290e0e53acccc64bb |
| رقم الأكسشن: | edsdoj.1ffbe2a79cb4a4290e0e53acccc64bb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 01448463 15734935 |
|---|---|
| DOI: | 10.1042/BSR20120023 |