دورية أكاديمية

Extracellular vesicles from bone marrow-derived mesenchymal stromal cells support ex vivo survival of human antibody secreting cells

التفاصيل البيبلوغرافية
العنوان: Extracellular vesicles from bone marrow-derived mesenchymal stromal cells support ex vivo survival of human antibody secreting cells
المؤلفون: Doan C. Nguyen, Holly C. Lewis, Chester Joyner, Vivien Warren, Haopeng Xiao, Haydn T. Kissick, Ronghu Wu, Jacques Galipeau, F. Eun-Hyung Lee
المصدر: Journal of Extracellular Vesicles, Vol 7, Iss 1 (2018)
بيانات النشر: Wiley, 2018.
سنة النشر: 2018
المجموعة: LCC:Cytology
مصطلحات موضوعية: Mesenchymal stromal cell, extracellular vesicles, antibody secretion cell, plasma cell, Cytology, QH573-671
الوصف: Extracellular vesicles (EVs) from bone marrow (BM)-derived mesenchymal stromal cells (BM-MSC) are novel mechanisms of cell-cell communication over short and long distances. BM-MSC have been shown to support human antibody secreting cells (ASC) survival ex vivo, but whether the crosstalk between the MSC-ASC interaction can occur via EVs is not known. Thus, we evaluated the role of EVs in ASC survival and IgG secretion. EVs were isolated from irradiated and non-irradiated primary BM-MSC and were quantified. They were further characterized by electron microscopy (EM) and CD63 and CD81 immuno-gold EM staining. Human ASC were isolated via fluorescence-activated cell sorting (FACS) and cultured ex vivo with the EV fractions, the EV-reduced fractions, or conventional media. IgG Elispots were used to measure the survival and functionality of the ASC. Contents of the EV fractions were evaluated by proteomics. We saw that both irradiated and non-irradiated MSC secretome preparations afforded vesicles of a size consistent with EVs. Both preparations appeared comparable in EM morphology and CD63 and CD81 immuno-gold EM. Both irradiated and non-irradiated EV fractions supported ASC function, at 88% and 90%, respectively, by day 3. In contrast, conventional media maintained only 4% ASC survival by day 3. To identify the specific factors that provided in vitro ASC support, we compared proteomes of the irradiated and non-irradiated EV fractions with conventional media. Pathway analysis of these proteins identified factors involved in the vesicle-mediated delivery of integrin signalling proteins. These findings indicate that BM-MSC EVs provide an effective support system for ASC survival and IgG secretion.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2001-3078
20013078
Relation: https://doaj.org/toc/2001-3078
DOI: 10.1080/20013078.2018.1463778
URL الوصول: https://doaj.org/article/ce20ad55404e4800851981a265d96979
رقم الأكسشن: edsdoj.20ad55404e4800851981a265d96979
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20013078
DOI:10.1080/20013078.2018.1463778