دورية أكاديمية
Stability of SARS-CoV-2 phylogenies.
العنوان: | Stability of SARS-CoV-2 phylogenies. |
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المؤلفون: | Yatish Turakhia, Nicola De Maio, Bryan Thornlow, Landen Gozashti, Robert Lanfear, Conor R Walker, Angie S Hinrichs, Jason D Fernandes, Rui Borges, Greg Slodkowicz, Lukas Weilguny, David Haussler, Nick Goldman, Russell Corbett-Detig |
المصدر: | PLoS Genetics, Vol 16, Iss 11, p e1009175 (2020) |
بيانات النشر: | Public Library of Science (PLoS), 2020. |
سنة النشر: | 2020 |
المجموعة: | LCC:Genetics |
مصطلحات موضوعية: | Genetics, QH426-470 |
الوصف: | The SARS-CoV-2 pandemic has led to unprecedented, nearly real-time genetic tracing due to the rapid community sequencing response. Researchers immediately leveraged these data to infer the evolutionary relationships among viral samples and to study key biological questions, including whether host viral genome editing and recombination are features of SARS-CoV-2 evolution. This global sequencing effort is inherently decentralized and must rely on data collected by many labs using a wide variety of molecular and bioinformatic techniques. There is thus a strong possibility that systematic errors associated with lab-or protocol-specific practices affect some sequences in the repositories. We find that some recurrent mutations in reported SARS-CoV-2 genome sequences have been observed predominantly or exclusively by single labs, co-localize with commonly used primer binding sites and are more likely to affect the protein-coding sequences than other similarly recurrent mutations. We show that their inclusion can affect phylogenetic inference on scales relevant to local lineage tracing, and make it appear as though there has been an excess of recurrent mutation or recombination among viral lineages. We suggest how samples can be screened and problematic variants removed, and we plan to regularly inform the scientific community with our updated results as more SARS-CoV-2 genome sequences are shared (https://virological.org/t/issues-with-sars-cov-2-sequencing-data/473 and https://virological.org/t/masking-strategies-for-sars-cov-2-alignments/480). We also develop tools for comparing and visualizing differences among very large phylogenies and we show that consistent clade- and tree-based comparisons can be made between phylogenies produced by different groups. These will facilitate evolutionary inferences and comparisons among phylogenies produced for a wide array of purposes. Building on the SARS-CoV-2 Genome Browser at UCSC, we present a toolkit to compare, analyze and combine SARS-CoV-2 phylogenies, find and remove potential sequencing errors and establish a widely shared, stable clade structure for a more accurate scientific inference and discourse. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1553-7390 1553-7404 |
Relation: | https://doaj.org/toc/1553-7390; https://doaj.org/toc/1553-7404 |
DOI: | 10.1371/journal.pgen.1009175 |
URL الوصول: | https://doaj.org/article/20e8f63f79c144ba9949393687772d59 |
رقم الأكسشن: | edsdoj.20e8f63f79c144ba9949393687772d59 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 15537390 15537404 |
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DOI: | 10.1371/journal.pgen.1009175 |