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Reconstruction of 3D structures of MET antibodies from electron microscopy 2D class averages.

التفاصيل البيبلوغرافية
العنوان: Reconstruction of 3D structures of MET antibodies from electron microscopy 2D class averages.
المؤلفون: Qi Chen, Michal Vieth, David E Timm, Christine Humblet, Dina Schneidman-Duhovny, Ilan E Chemmama, Andrej Sali, Wei Zeng, Jirong Lu, Ling Liu
المصدر: PLoS ONE, Vol 12, Iss 4, p e0175758 (2017)
بيانات النشر: Public Library of Science (PLoS), 2017.
سنة النشر: 2017
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Dynamics of three MET antibody constructs (IgG1, IgG2, and IgG4) and the IgG4-MET antigen complex was investigated by creating their atomic models with an integrative experimental and computational approach. In particular, we used two-dimensional (2D) Electron Microscopy (EM) images, image class averaging, homology modeling, Rapidly exploring Random Tree (RRT) structure sampling, and fitting of models to images, to find the relative orientations of antibody domains that are consistent with the EM images. We revealed that the conformational preferences of the constructs depend on the extent of the hinge flexibility. We also quantified how the MET antigen impacts on the conformational dynamics of IgG4. These observations allow to create testable hypothesis to investigate MET biology. Our protocol may also help describe structural diversity of other antigen systems at approximately 5 Å precision, as quantified by Root-Mean-Square Deviation (RMSD) among good-scoring models.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC5391116?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0175758
URL الوصول: https://doaj.org/article/20ed679d31d2479d8afc55306b1b8479
رقم الأكسشن: edsdoj.20ed679d31d2479d8afc55306b1b8479
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0175758