دورية أكاديمية
أعرض في EDS

Nodeomics: pathogen detection in vertebrate lymph nodes using meta-transcriptomics.

التفاصيل البيبلوغرافية
العنوان: Nodeomics: pathogen detection in vertebrate lymph nodes using meta-transcriptomics.
المؤلفون: Nicola E Wittekindt, Abinash Padhi, Stephan C Schuster, Ji Qi, Fangqing Zhao, Lynn P Tomsho, Lindsay R Kasson, Michael Packard, Paul Cross, Mary Poss
المصدر: PLoS ONE, Vol 5, Iss 10, p e13432 (2010)
بيانات النشر: Public Library of Science (PLoS), 2010.
سنة النشر: 2010
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: The ongoing emergence of human infections originating from wildlife highlights the need for better knowledge of the microbial community in wildlife species where traditional diagnostic approaches are limited. Here we evaluate the microbial biota in healthy mule deer (Odocoileus hemionus) by analyses of lymph node meta-transcriptomes. cDNA libraries from five individuals and two pools of samples were prepared from retropharyngeal lymph node RNA enriched for polyadenylated RNA and sequenced using Roche-454 Life Sciences technology. Protein-coding and 16S ribosomal RNA (rRNA) sequences were taxonomically profiled using protein and rRNA specific databases. Representatives of all bacterial phyla were detected in the seven libraries based on protein-coding transcripts indicating that viable microbiota were present in lymph nodes. Residents of skin and rumen, and those ubiquitous in mule deer habitat dominated classifiable bacterial species. Based on detection of both rRNA and protein-coding transcripts, we identified two new proteobacterial species; a Helicobacter closely related to Helicobacter cetorum in the Helicobacter pylori/Helicobacter acinonychis complex and an Acinetobacter related to Acinetobacter schindleri. Among viruses, a novel gamma retrovirus and other members of the Poxviridae and Retroviridae were identified. We additionally evaluated bacterial diversity by amplicon sequencing the hypervariable V6 region of 16S rRNA and demonstrate that overall taxonomic diversity is higher with the meta-transcriptomic approach. These data provide the most complete picture to date of the microbial diversity within a wildlife host. Our research advances the use of meta-transcriptomics to study microbiota in wildlife tissues, which will facilitate detection of novel organisms with pathogenic potential to human and animals.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC2956653?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0013432
URL الوصول: https://doaj.org/article/21865c0a7bee4e8ba4f09dcaa52404dc
رقم الأكسشن: edsdoj.21865c0a7bee4e8ba4f09dcaa52404dc
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0013432