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Molecular threading: mechanical extraction, stretching and placement of DNA molecules from a liquid-air interface.

التفاصيل البيبلوغرافية
العنوان: Molecular threading: mechanical extraction, stretching and placement of DNA molecules from a liquid-air interface.
المؤلفون: Andrew C Payne, Michael Andregg, Kent Kemmish, Mark Hamalainen, Charlotte Bowell, Andrew Bleloch, Nathan Klejwa, Wolfgang Lehrach, Ken Schatz, Heather Stark, Adam Marblestone, George Church, Christopher S Own, William Andregg
المصدر: PLoS ONE, Vol 8, Iss 7, p e69058 (2013)
بيانات النشر: Public Library of Science (PLoS), 2013.
سنة النشر: 2013
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: We present "molecular threading", a surface independent tip-based method for stretching and depositing single and double-stranded DNA molecules. DNA is stretched into air at a liquid-air interface, and can be subsequently deposited onto a dry substrate isolated from solution. The design of an apparatus used for molecular threading is presented, and fluorescence and electron microscopies are used to characterize the angular distribution, straightness, and reproducibility of stretched DNA deposited in arrays onto elastomeric surfaces and thin membranes. Molecular threading demonstrates high straightness and uniformity over length scales from nanometers to micrometers, and represents an alternative to existing DNA deposition and linearization methods. These results point towards scalable and high-throughput precision manipulation of single-molecule polymers.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3729692?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0069058
URL الوصول: https://doaj.org/article/a2186a4f4c66421582d57bd1fe42a690
رقم الأكسشن: edsdoj.2186a4f4c66421582d57bd1fe42a690
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0069058