دورية أكاديمية
Qualitative and quantitative analysis of glucosinolates and nucleosides in Radix Isatidis by HPLC and liquid chromatography tandem mass spectrometry
| العنوان: | Qualitative and quantitative analysis of glucosinolates and nucleosides in Radix Isatidis by HPLC and liquid chromatography tandem mass spectrometry |
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| المؤلفون: | Xiuming Wang, Yuanyuan Xie, Xiaomei Hu, Youkang Li, Ping Hu, Yiming Wang, Qionglin Liang, Guoan Luo |
| المصدر: | Acta Pharmaceutica Sinica B, Vol 3, Iss 5, Pp 337-344 (2013) |
| بيانات النشر: | Elsevier, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | Radix Isatidis, Glucosinolate, Nucleoside, R,S-Goitrin, Quality control, HPLC–DAD–ESI/MS, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Multi-component fingerprinting and quantitation of the glucosinolates and nucleosides in samples of Radix Isatidis have been carried out using high-performance liquid chromatography with diode-array detection and electrospray ionization tandem mass spectrometry (HPLC–DAD–ESI/MS). Five nucleosides together with one glucosinolate were identified by comparing retention times, ultraviolet spectra, mass spectra and/or empirical molecular formulae of reference compounds. Quantitation of these six compounds was carried out simultaneously by HPLC on a Phenomenex Luna C18 column using gradient elution with methanol and water and detection at 254 nm. All calibration curves were linear (r>0.9994) within test ranges. Limits of detection and quantitation were 0.33 ng and 2.50 ng on column, respectively. Intra- and inter-day precision (as relative standard deviation) for all analytes was |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2211-3835 2211-3843 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2211383513000786; https://doaj.org/toc/2211-3835; https://doaj.org/toc/2211-3843 |
| DOI: | 10.1016/j.apsb.2013.08.002 |
| URL الوصول: | https://doaj.org/article/21a8121729e04084baec380a0cc38b81 |
| رقم الأكسشن: | edsdoj.21a8121729e04084baec380a0cc38b81 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22113835 22113843 |
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| DOI: | 10.1016/j.apsb.2013.08.002 |