دورية أكاديمية
Identification of Key Genes Related to Postnatal Testicular Development Based on Transcriptomic Data of Testis in Hu Sheep
| العنوان: | Identification of Key Genes Related to Postnatal Testicular Development Based on Transcriptomic Data of Testis in Hu Sheep |
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| المؤلفون: | Haiyue Xu, Wu Sun, Shengwei Pei, Wanhong Li, Fadi Li, Xiangpeng Yue |
| المصدر: | Frontiers in Genetics, Vol 12 (2022) |
| بيانات النشر: | Frontiers Media S.A., 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Genetics |
| مصطلحات موضوعية: | sheep, testis, transcriptome, DEGs, male reproduction, Genetics, QH426-470 |
| الوصف: | The selection of testis size can improve the reproductive capacity of livestock used for artificial insemination and has been considered as an important strategy for accelerating the breeding process. Although much work has been done to investigate the mechanisms of testis development in various species, there is little information available in regard to the differences in transcriptomic profiling of sheep testes at different developmental stages. In this work, we aimed to identify differentially expressed genes (DEGs) by RNA-Seq in sheep during different growth stages, including 0 month old (infant, M0), 3 months old (puberty, M3), 6 months old (sexual maturity, M6) and 12 months old (body maturity, M12). A total of 4,606 (2,381 up and 2,225 down), 7,500 (4,368 up and 3,132 down), 15 (8 up and seven down) DEGs were identified in M3_vs_M0, M6_vs_M3, and M12_vs_M6 comparison, respectively. Of which, a number of genes were continuously up-regulated and down-regulated with testicular development, including ODF3, ZPBP1, PKDREJ, MYBL1, PDGFA, IGF1, LH, INSL3, VIM, AMH, INHBA, COL1A1, COL1A2, and INHA. GO analysis illustrated that DEGs were mainly involved in testis development and spermatogenesis. KEGG analysis identified several important pathways and verified several reproduction-associated DEGs such as COL1A1, COL1A2, PDGFA, and IGF1. In addition, two gene modules highly associated with testis development and core genes with testis size were identified using weighted gene co-expression network analysis (WGCNA), including hub genes positively associated with testis size such as RANBP9, DNAH17, SPATA4, CIB4 and SPEM1, and those negatively associated with testis size such as CD81, CSK, PDGFA, VIM, and INHBA. This study comprehensively identified key genes related to sheep testicular development, which may provide potential insights for understanding male fertility and better guide in animal breeding. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1664-8021 81826184 |
| Relation: | https://www.frontiersin.org/articles/10.3389/fgene.2021.773695/full; https://doaj.org/toc/1664-8021 |
| DOI: | 10.3389/fgene.2021.773695 |
| URL الوصول: | https://doaj.org/article/2224e81826184f4faeb52694b2f53be7 |
| رقم الأكسشن: | edsdoj.2224e81826184f4faeb52694b2f53be7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16648021 81826184 |
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| DOI: | 10.3389/fgene.2021.773695 |