دورية أكاديمية
Holographic Recording of Unslanted Volume Transmission Gratings in Acrylamide/Propargyl Acrylate Hydrogel Layers: Towards Nucleic Acids Biosensing
| العنوان: | Holographic Recording of Unslanted Volume Transmission Gratings in Acrylamide/Propargyl Acrylate Hydrogel Layers: Towards Nucleic Acids Biosensing |
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| المؤلفون: | Paola Zezza, María Isabel Lucío, Izabela Naydenova, María-José Bañuls, Ángel Maquieira |
| المصدر: | Gels, Vol 9, Iss 9, p 710 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Science LCC:Chemistry LCC:Inorganic chemistry LCC:General. Including alchemy |
| مصطلحات موضوعية: | holographic recording, volume transmission grating, hydrogel layer, diffraction efficiency, biosensor, Science, Chemistry, QD1-999, Inorganic chemistry, QD146-197, General. Including alchemy, QD1-65 |
| الوصف: | The role of volume hydrogel holographic gratings as optical transducers in sensor devices for point-of-care applications is increasing due to their ability to be functionalized for achieving enhanced selectivity. The first step in the development of these transducers is the optimization of the holographic recording process. The optimization aims at achieving gratings with reproducible diffraction efficiency, which remains stable after reiterative washings, typically required when working with analytes of a biological nature or several step tests. The recording process of volume phase transmission gratings within Acrylamide/Propargyl Acrylate hydrogel layers reported in this work was successfully performed, and the obtained diffraction gratings were optically characterized. Unslanted volume transmission gratings were recorded in the hydrogel layers diffraction efficiencies; up to 80% were achieved. Additionally, the recorded gratings demonstrated stability in water after multiple washing steps. The hydrogels, after functionalization with oligonucleotide probes, yields a specific hybridization response, recognizing the complementary strand as demonstrated by fluorescence. Analyte-sensitive hydrogel layers with holographic structures are a promising candidate for the next generation of in vitro diagnostic tests. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2310-2861 76576264 |
| Relation: | https://www.mdpi.com/2310-2861/9/9/710; https://doaj.org/toc/2310-2861 |
| DOI: | 10.3390/gels9090710 |
| URL الوصول: | https://doaj.org/article/23d4c76576264974ac572a0b2b8738a9 |
| رقم الأكسشن: | edsdoj.23d4c76576264974ac572a0b2b8738a9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23102861 76576264 |
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| DOI: | 10.3390/gels9090710 |