The present investigation was undertaken to assess the spectrum of chlorophyll mutation in Hibiscus rosa-sinensis L. Cultivar Red Single and also to study the mutagenic effectiveness and efficiency in M1V1 generation of Hibiscus rosa-sinensis L. Cultivar Red Single. Semi-hardwood cuttings of Hibiscus were treated with three doses of gamma rays viz., 25, 30 and 35 Gy and three doses of EMS viz., 0.8% (64.43 mM), 0.9% (72.48 mM, 1.0% (80.54 mM) separately. Physical mutagens created a high frequency as well as a wide spectrum of chlorophyll mutants. Totally five types of chlorophyll mutant’s viz., albino, xantha, viridis, chlorina and xantha-viridis were observed as a result of physical and chemical mutation. The mutagenic effectiveness and efficiency were calculated based on biological damage as well as chlorophyll mutation frequency on M1V1 plants. Mutagenic efficiency and effectiveness were higher in gamma radiation-induced plants particularly in a lower dose of 25 Gy. The highest mutation rate in terms of effectiveness and efficiency was observed in gamma rays than the Ethyl Methane Sulphonate treatment.
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