دورية أكاديمية
Abnormal Sensory Protein Expression and Urothelial Dysfunction in Ketamine-Related Cystitis in Humans
| العنوان: | Abnormal Sensory Protein Expression and Urothelial Dysfunction in Ketamine-Related Cystitis in Humans |
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| المؤلفون: | Yao Chou Tsai, Lori Birder, Hann-Chorng Kuo |
| المصدر: | International Neurourology Journal, Vol 20, Iss 3, Pp 197-202 (2016) |
| بيانات النشر: | Korean Continence Society, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Diseases of the genitourinary system. Urology |
| مصطلحات موضوعية: | Ketamine, Urothelium, Diseases of the genitourinary system. Urology, RC870-923 |
| الوصف: | Purpose The aim of this study was to analyze patterns of sensory protein expression and urothelial dysfunction in ketamine-related cystitis (KC) in humans. Methods Biopsies of bladder mucosa were performed in 29 KC patients during cystoscopy. Then specimens were analyzed for tryptase, zonula occludens-1 (ZO-1), E-cadherin, and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) with immunofluorescence staining and quantification. In addition, 10 healthy control bladder specimens were analyzed and compared with the KC specimens. Another 16 whole bladder specimens obtained from partial cystectomy were also analyzed for the muscarinic receptors M2 and M3, endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), β-3 adrenergic receptors (β3-ARs), and the P2X3 receptor by western blotting. In addition, 3 normal control bladder specimens were analyzed and compared with the KC specimens. Results The KC bladder mucosa revealed significantly less expression of ZO-1 and E-cadherin, and greater expression of TUNEL and tryptase activity than the control samples. The expression of M3 and β3-AR in the KC specimens was significantly greater than in the controls. The expression of iNOS, eNOS, M2, and P2X3 was not significantly different between the KC and control specimens. Conclusions The bladder tissue of KC patients revealed significant urothelial dysfunction, which was associated with mast-cell mediated inflammation, increased urothelial cell apoptosis, and increased expression of the M3 and β3-AR. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2093-4777 2093-6931 |
| Relation: | http://www.einj.org/upload/pdf/inj-1632634-317.pdf; https://doaj.org/toc/2093-4777; https://doaj.org/toc/2093-6931 |
| DOI: | 10.5213/inj.1632634.317 |
| URL الوصول: | https://doaj.org/article/24b869e45b1042ddb1a1ce0b748db7db |
| رقم الأكسشن: | edsdoj.24b869e45b1042ddb1a1ce0b748db7db |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20934777 20936931 |
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| DOI: | 10.5213/inj.1632634.317 |