دورية أكاديمية
Effect of time and autologous serum addition on the analysis of cerebrospinal fluid in horses
| العنوان: | Effect of time and autologous serum addition on the analysis of cerebrospinal fluid in horses |
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| المؤلفون: | Camilla Quattrini, Rebeca Scalco, William Vernau, Pouya Dini, Monica Aleman |
| المصدر: | Journal of Veterinary Internal Medicine, Vol 37, Iss 2, Pp 713-717 (2023) |
| بيانات النشر: | Wiley, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Veterinary medicine |
| مصطلحات موضوعية: | autologous serum, cerebro, CSF, horse, spinal fluid, Veterinary medicine, SF600-1100 |
| الوصف: | Abstract Background Cerebrospinal fluid (CSF) is highly labile and delayed processing might alter results of analysis. Hypothesis/Objectives To determine the effects of time and addition of autologous serum on cytological evaluation of CSF. Animals Ten client‐owned adult horses requiring euthanasia. Methods Prospective study. Serum and CSF were collected from each horse before and within 10 minutes after euthanasia. CSF samples were divided into 15 aliquots (2 mL each); 1 aliquot was submitted for routine CSF analysis within 60 minutes of collection. Four drops of autologous serum were added to 7 of the aliquots, and stored at 4°C (serum group); the remaining 7 samples were stored unaltered at 4°C (control group). Total nucleated cell count (TNCC) and cell morphology score were done at T4, T8, T12, T24, T48, T72, and T96 hours after collection. Protein concentration was measured in the control group at T0 and T96 hours. Results The cell morphology scores were significantly different in the control group at T48 (median 2, range 0‐4), T72 (2, 0‐4), and T96 (3, 0‐4) in comparison to T0 (1). No change was observed in the serum group. TNCC remained stable over time in both groups. No statistically significant difference in CSF protein concentration was found between T0 and T96. Conclusions and Clinical Importance The addition of autologous serum to an aliquot of CSF sample before shipping improves the preservation of cell morphology up to 96 hours after collection. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1939-1676 0891-6640 |
| Relation: | https://doaj.org/toc/0891-6640; https://doaj.org/toc/1939-1676 |
| DOI: | 10.1111/jvim.16637 |
| URL الوصول: | https://doaj.org/article/24f246e2d2574baa8c697b6167e14545 |
| رقم الأكسشن: | edsdoj.24f246e2d2574baa8c697b6167e14545 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19391676 08916640 |
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| DOI: | 10.1111/jvim.16637 |