دورية أكاديمية
miR-30e-5p and miR-15a Synergistically Regulate Fatty Acid Metabolism in Goat Mammary Epithelial Cells via LRP6 and YAP1
العنوان: | miR-30e-5p and miR-15a Synergistically Regulate Fatty Acid Metabolism in Goat Mammary Epithelial Cells via LRP6 and YAP1 |
---|---|
المؤلفون: | Zhi Chen, Huiling Qiu, Liuan Ma, Jun Luo, Shuang Sun, Kang Kang, Deming Gou, Juan J. Loor |
المصدر: | International Journal of Molecular Sciences, Vol 17, Iss 11, p 1909 (2016) |
بيانات النشر: | MDPI AG, 2016. |
سنة النشر: | 2016 |
المجموعة: | LCC:Biology (General) LCC:Chemistry |
مصطلحات موضوعية: | miR-30e-5p, miR-15a, fat metabolism, LRP6, YAP1, Biology (General), QH301-705.5, Chemistry, QD1-999 |
الوصف: | MicroRNA (miRNA) regulates the expression of genes and influences a series of biological processes, including fatty acid metabolism. We screened the expression of miRNA in goat mammary glands during peak-lactation and non-lactating (“dry”) periods, and performed an in vitro study with goat mammary epithelial cells (GMEC) prior to sequencing analysis. Results illustrated that miR-30e-5p and miR-15a were highly expressed. Utilizing a luciferase reporter assay and Western blot, low-density lipoprotein receptor-related protein 6 (LRP6) and Yes associated protein 1 (YAP1) genes were demonstrated to be a target of miR-30e-5p and miR-15a in GMEC. Moreover, we demonstrated that the overexpression of miR-30e-5p and miR-15a in GMEC promoted fat metabolism while their knockdown impaired fat metabolism. These findings extend the discovery of a key role of miR-30e-5p and miR-15a in mediating adipocyte differentiation by suggesting a role in promoting milk fat synthesis. In conclusion, our findings indicate that miR-30e-5p, together with miR-15a, represses expression of LRP6 and promotes fat metabolism in GMEC. The data expanded our knowledge on the function of miRNAs in milk fat metabolism and synthesis in ruminant mammary cells. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1422-0067 |
Relation: | http://www.mdpi.com/1422-0067/17/11/1909; https://doaj.org/toc/1422-0067 |
DOI: | 10.3390/ijms17111909 |
URL الوصول: | https://doaj.org/article/d252232d5ca5489aa14f7cf4b493f17c |
رقم الأكسشن: | edsdoj.252232d5ca5489aa14f7cf4b493f17c |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 14220067 |
---|---|
DOI: | 10.3390/ijms17111909 |