دورية أكاديمية
Mechanisms underlying the improvement of preeclampsia through salvianolic acid B-regulated miRNA-155/CXCR4
| العنوان: | Mechanisms underlying the improvement of preeclampsia through salvianolic acid B-regulated miRNA-155/CXCR4 |
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| المؤلفون: | Chuanlian Zhang, Xue Han, Xiaoxue Wang |
| المصدر: | Archives of Medical Science, Vol 19, Iss 2, Pp 430-447 (2020) |
| بيانات النشر: | Termedia Publishing House, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | sal b, preeclampsia, htr8/svneo cell, mirna-155, cxcr4, p-akt, salvia miltiorrhiza, Medicine |
| الوصف: | Introduction The aim of this study was to evaluate the effects and mechanisms of salvianolic acid B (Sal B) in preeclampsia treatment by in vivo and in vitro study. Material and methods Rats were randomly divided into 5 groups. In order to establish the model of preeclampsia, endotoxin was administered to the rats in the Sal B intervention and model groups. The systolic blood pressure (SBP) of the tail artery and urine protein concentration were observed at different points, the miRNA-155 and CXCR4 gene expression levels by RT-PCR and the CXCR4 and p-AKT protein expression by WB assay. Using HTR8/SVneo to explain the mechanisms; evaluating the miRNA-155 and CXCR4 mRNA expression by RT-PCR assay, measuring the cell invasion and migration by transwell and wound healing assay in different groups; evaluating the CXCR4 and p-AKT protein expression by WB assay and p-AKT nucleation volume by cellular immunofluorescence were evaluated. Results Compared with the normal group, the systolic blood pressure and urine protein were significantly increased in the model group (p < 0.05), serum NO concentration was significantly down-regulated (all p < 0.05), CXCR4 and miRNA-155 mRNA expression was significantly different and CXCR4 and p-AKT protein expression was significantly suppressed (all p < 0.05). With Sal B supplement, the SBP, urine protein and NO concentration were significantly improved with dose-dependent (all p < 0.05). In the cell experiment, the cell invasion and migration ability were significantly improved with Sal B supplement (both p < 0.05). However, with miRNA-155 transfection, the cell invasion and migration ability were suppressed with Sal B treatment (both p < 0.05). Conclusions Sal B improved preeclampsia via regulation of miRNA-155/CXCR4 in the in vitro and vivo study. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1734-1922 1896-9151 |
| Relation: | https://www.archivesofmedicalscience.com/Mechanisms-underlying-the-improvement-of-preeclampsia-through-salvianolic-acid-B,110947,0,2.html; https://doaj.org/toc/1734-1922; https://doaj.org/toc/1896-9151 |
| DOI: | 10.5114/aoms.2020.92938 |
| URL الوصول: | https://doaj.org/article/257eaf04dd614c4a8fcf3637c75d9b6d |
| رقم الأكسشن: | edsdoj.257eaf04dd614c4a8fcf3637c75d9b6d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17341922 18969151 |
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| DOI: | 10.5114/aoms.2020.92938 |