دورية أكاديمية
Inhibitory effect of THPGV-0 on the histamine release from antigen-induced RBL-2H3 cells
| العنوان: | Inhibitory effect of THPGV-0 on the histamine release from antigen-induced RBL-2H3 cells |
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| المؤلفون: | Agung Endro Nugroho, Ritmaleni Ritmaleni, Novrizal Abdi Sahid, Kazutaka Maeyama |
| المصدر: | Indonesian Journal of Pharmacy, Vol 21, Iss 4, Pp 242-249 (2010) |
| بيانات النشر: | Universitas Gadjah Mada, 2010. |
| سنة النشر: | 2010 |
| المجموعة: | LCC:Pharmacy and materia medica |
| مصطلحات موضوعية: | tetrahydropentagamavunon-0, allergy, histamine, RBL-2H3 cells, Pharmacy and materia medica, RS1-441 |
| الوصف: | Tetrahydropentagamavunon-0 (THPGV-0) is assumed to be main metabolite product of biotransformation process of PGV-0. THPGV-0 was synthesized by converting PGV-0 to the compound by hydrogenation with Pd/C as a catalyst. PGV-0 potently inhibited the histamine release from rat mast cells in vitro and in vivo, however, ironically only traces amount of compound was found in the blood. THPGV-0 is assumed to have important roles in the biological effects of PGV-0 in vivo. In present study, we investigated the antiallergy effect of THPGV-0 in compare to this of PGV-0 in vitro. The study was performed by using rat basophilic leukemia (RBL-2H3) cell line, a tumor analog of mucosal mast cells. DNP-BSA, an antigen, was used as an inducer for stimulating the histamine release from mast cells. In present study, THPGV-0 at low concentration did not succeed to inhibit the histamine release, but at higher concentration (30 and 100 M) showed strong effects. THPGV-0 at concentration of 100 M depleted the histamine release by 96.10 0.51%. In compare to PGV-0, THPGV-0 has higher efficacy but less potent. In the study, the possibilities of the spontaneous release from RBL-2H3 cells by the compounds were also observed. All concentrations of THPGV-0 as well as PGV-0 showed low spontaneous histamine release, less than 10 % of the total histamine contained in RBL-2H3 cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2338-9427 2338-9486 |
| Relation: | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/474; https://doaj.org/toc/2338-9427; https://doaj.org/toc/2338-9486 |
| DOI: | 10.14499/indonesianjpharm0iss0pp242-249 |
| URL الوصول: | https://doaj.org/article/25e2311482ff428eacd980a67335f1c5 |
| رقم الأكسشن: | edsdoj.25e2311482ff428eacd980a67335f1c5 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 23389427 23389486 |
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| DOI: | 10.14499/indonesianjpharm0iss0pp242-249 |