دورية أكاديمية
Pluripotent stem cells secrete Activin A to improve their epiblast competency after injection into recipient embryos
| العنوان: | Pluripotent stem cells secrete Activin A to improve their epiblast competency after injection into recipient embryos |
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| المؤلفون: | Jinzhu Xiang, Suying Cao, Liang Zhong, Hanning Wang, Yangli Pei, Qingqing Wei, Bingqiang Wen, Haiyuan Mu, Shaopeng Zhang, Liang Yue, Genhua Yue, Bing Lim, Jianyong Han |
| المصدر: | Protein & Cell, Vol 9, Iss 8, Pp 717-728 (2017) |
| بيانات النشر: | Oxford University Press, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Cytology LCC:Animal biochemistry |
| مصطلحات موضوعية: | pluripotent stem cells, 4-cell embryo injection, secreted proteins, Activin A, chimeric mice, Cytology, QH573-671, Animal biochemistry, QP501-801 |
| الوصف: | Abstract It is not fully clear why there is a higher contribution of pluripotent stem cells (PSCs) to the chimera produced by injection of PSCs into 4-cell or 8-cell stage embryos compared with blastocyst injection. Here, we show that not only embryonic stem cells (ESCs) but also induced pluripotent stem cells (iPSCs) can generate F0 nearly 100% donor cell-derived mice by 4-cell stage embryo injection, and the approach has a “dose effect”. Through an analysis of the PSC-secreted proteins, Activin A was found to impede epiblast (EPI) lineage development while promoting trophectoderm (TE) differentiation, resulting in replacement of the EPI lineage of host embryos with PSCs. Interestingly, the injection of ESCs into blastocysts cultured with Activin A (cultured from 4-cell stage to early blastocyst at E3.5) could increase the contribution of ESCs to the chimera. The results indicated that PSCs secrete protein Activin A to improve their EPI competency after injection into recipient embryos through influencing the development of mouse early embryos. This result is useful for optimizing the chimera production system and for a deep understanding of PSCs effects on early embryo development. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1674-800X 1674-8018 |
| Relation: | http://link.springer.com/article/10.1007/s13238-017-0470-y; https://doaj.org/toc/1674-800X; https://doaj.org/toc/1674-8018 |
| DOI: | 10.1007/s13238-017-0470-y |
| URL الوصول: | https://doaj.org/article/c260dcbb9fca48ddb41cb7f5f5230338 |
| رقم الأكسشن: | edsdoj.260dcbb9fca48ddb41cb7f5f5230338 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1674800X 16748018 |
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| DOI: | 10.1007/s13238-017-0470-y |