The stingless bee, Tetragonula sapiens, is one of the species producing propolis in Indonesia. To date, there is still limited information about the standard characteristics and biological activity of propolis. This study investigated the standardized extract of Indonesian propolis in terms of its potency for controlling diabetes and high blood pressure in vitro, in accordance with its standard parameters. Three types of extracts; i.e., ethanolic extract of propolis (EEP), spray-dry propolis without wax (SDPNW), and spray-dry propolis with wax (SDPW); were prepared from T. sapiens stingless bees collected in South Sulawesi, Indonesia. The ethanolic extractable, loss on drying, ash, and water content parameters of EEP, SDPNW, and SDPW met the natural extract requirement. There is no significant value for their residual solvent levels (
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