دورية أكاديمية
Qualitative and Quantitative Comparison of Plasma Exosomes from Neonates and Adults
| العنوان: | Qualitative and Quantitative Comparison of Plasma Exosomes from Neonates and Adults |
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| المؤلفون: | Julia Peñas-Martínez, María N. Barrachina, Ernesto José Cuenca-Zamora, Ginés Luengo-Gil, Susana Belén Bravo, Eva Caparrós-Pérez, Raúl Teruel-Montoya, José Eliseo-Blanco, Vicente Vicente, Ángel García, Irene Martínez-Martínez, Francisca Ferrer-Marín |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 4, p 1926 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | exosomes, neonatal platelets, platelet transfusion, protein S, proteomic, von Willebrand factor, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Exosomes are extracellular vesicles that contain nucleic acids, lipids and metabolites, and play a critical role in health and disease as mediators of intercellular communication. The majority of extracellular vesicles in the blood are platelet-derived. Compared to adults, neonatal platelets are hyporeactive and show impaired granule release, associated with defects in Soluble N-ethylmaleimide-sensitive fusion Attachment protein REceptor (SNARE) proteins. Since these proteins participate in biogenesis of exosomes, we investigated the potential differences between newborn and adult plasma-derived exosomes. Plasma-derived exosomes were isolated by ultracentrifugation of umbilical cord blood from full-term neonates or peripheral blood from adults. Exosome characterization included size determination by transmission electron microscopy and quantitative proteomic analysis. Plasma-derived exosomes from neonates were significantly smaller and contained 65% less protein than those from adults. Remarkably, 131 proteins were found to be differentially expressed, 83 overexpressed and 48 underexpressed in neonatal (vs. adult) exosomes. Whereas the upregulated proteins in plasma exosomes from neonates are associated with platelet activation, coagulation and granule secretion, most of the underexpressed proteins are immunoglobulins. This is the first study showing that exosome size and content change with age. Our findings may contribute to elucidating the potential “developmental hemostatic mismatch risk” associated with transfusions containing plasma exosomes from adults. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/22/4/1926; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms22041926 |
| URL الوصول: | https://doaj.org/article/26d7807a6c9c4b95a5db83b799d1880b |
| رقم الأكسشن: | edsdoj.26d7807a6c9c4b95a5db83b799d1880b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms22041926 |