Summary: Long-term continuous electroencephalogram (EEG) monitoring is crucial for neuroengineering but suffers from hardware limitations. Here, we present a protocol for EEG recording using a long-term stable and reagent-free-cross-linked hydrogel with configurable mechanical and adhesive properties. We describe steps for fabricating the hydrogel and performing material characterizations and stability tests. We detail procedures for setting up the EEG recording configuration and data analysis. This protocol can facilitate EEG recording experiments with the hydrogel, as well as other novel materials and devices.For complete details on the use and execution of this protocol, please refer to Hsieh et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
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