دورية أكاديمية
Carboxymethyl cellulase production from a Paenibacillus sp.
| العنوان: | Carboxymethyl cellulase production from a Paenibacillus sp. |
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| المؤلفون: | Mihajlovski Katarina R., Davidović Slađana Z., Carević Milica B., Radovanović Neda R., Šiler-Marinković Slavica S., Rajilić-Stojanović Mirjana D., Dimitrijević-Branković Suzana I. |
| المصدر: | Hemijska Industrija, Vol 70, Iss 3, Pp 329-338 (2016) |
| بيانات النشر: | Association of Chemical Engineers of Serbia, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Chemical technology |
| مصطلحات موضوعية: | Paenibacillus sp., cellulose, CMCase production, optimal conditions, Chemical technology, TP1-1185 |
| الوصف: | Cellulases are industrially important enzymes with a potential to convert cellulose into fermentable sugars. Novel bacterial isolate Paenibacillus sp. CKS1 was tested for cellulase activity and the optimal conditions for carboxymethyl cellulase (CMCase) production were determined. Maximum CMCase activity was obtained in the third passage of the bacterial culture after 3 days of incubation at 30°C. Cellobiose and yeast extract was the optimal source of carbon and nitrogen for induction of CMCase activity. In addition, with initial pH 7 of the medium and 40 ml of working volume in 500 ml culture flasks with shaking at 150 rpm, the maximum CMCase activity in a crude culture supernatant reached value of 0.532±0.006 U/ml. For crude CMCase, optimal temperature was 50°C and optimal pH 4.8, respectively. HPLC analysis confirmed the bacterium is capable to hydrolise CMC to glucose and other soluble sugars. [Projekat Ministarstva nauke Republike Srbije, br. TR 31035] |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Serbian |
| تدمد: | 0367-598X 2217-7426 |
| Relation: | https://doaj.org/toc/0367-598X; https://doaj.org/toc/2217-7426 |
| DOI: | 10.2298/HEMIND150222038M |
| URL الوصول: | https://doaj.org/article/d2712bf46e5b409cb0ba8fca941582c5 |
| رقم الأكسشن: | edsdoj.2712bf46e5b409cb0ba8fca941582c5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 0367598X 22177426 |
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| DOI: | 10.2298/HEMIND150222038M |