دورية أكاديمية
Influence of the Photodegradation of Azathioprine on DNA and Cells
| العنوان: | Influence of the Photodegradation of Azathioprine on DNA and Cells |
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| المؤلفون: | Mihaela-Cristina Bunea, Victor-Constantin Diculescu, Monica Enculescu, Daniela Oprea, Teodor Adrian Enache |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 22, p 14438 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | azathioprine, photodegradation, spectrophotometry, voltammetry, L929, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Azathioprine (AZA) is a pharmacologic immunosuppressive agent administrated in various conditions such as autoimmune disease or to prevent the rejection of organ transplantation. The mechanism of action is based on its biologically active metabolite 6-mercaptopurine (6-MP), which is converted, among others, into thioguanine nucleotides capable of incorporating into replicating DNA, which may act as a strong UV chromophore and trigger DNA oxidation. The interaction between azathioprine and DNA, before and after exposure to solar simulator radiation, was investigated using UV–vis spectrometry and differential pulse voltammetry at a glassy carbon electrode. The results indicated that the interaction of AZA with UV radiation was pH-dependent and occurred with the formation of several metabolites, which induced oxidative damage in DNA, and the formation of DNA-metabolite adducts. Moreover, the viability assays obtained for the L929 cell culture showed that both azathioprine and degraded azathioprine induced a decrease in cell proliferation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/23/22/14438; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms232214438 |
| URL الوصول: | https://doaj.org/article/e276f0a2aee64a749efb2280072c07a9 |
| رقم الأكسشن: | edsdoj.276f0a2aee64a749efb2280072c07a9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms232214438 |