دورية أكاديمية
Evaluation of novel surfactants for plant transformation
| العنوان: | Evaluation of novel surfactants for plant transformation |
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| المؤلفون: | Jennie Huynh, Sara K. Hotton, Ron Chan, Yasra Syed, James Thomson |
| المصدر: | BMC Research Notes, Vol 15, Iss 1, Pp 1-5 (2022) |
| بيانات النشر: | BMC, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine LCC:Biology (General) LCC:Science (General) |
| مصطلحات موضوعية: | Transformation, Surfactant, Arabidopsis, Agrobacterium, Silwet® L-77, BREAK-THRU®-OE446, -S200, -S233, -S240, -S279, -S301 and -SP133, Medicine, Biology (General), QH301-705.5, Science (General), Q1-390 |
| الوصف: | Abstract Objective Assess the efficiency of seven novel surfactants, relative to the typical Silwet® L-77, for floral dip transformation of Arabidopsis. Results Floral dip transformation of Arabidopsis has been used consistently for 20 years with little change in the protocol. Here we directly compare seven novel surfactants (BREAK-THRU®-OE446, S200, S233, S240, S279, S301 and SP133) to the standard Silwet® L-77 for efficiency of Arabidopsis transformation providing an example of how the surfactants can help other plant transformation protocols. Relative transformation efficiencies ranged from − 44 to + 45% compared to Silwet® L-77. Surfactants S200, S240, and S279 demonstrated the greatest enhancement in transformation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1756-0500 |
| Relation: | https://doaj.org/toc/1756-0500 |
| DOI: | 10.1186/s13104-022-06251-5 |
| URL الوصول: | https://doaj.org/article/2778c5793cf44aa0a4c9ee0d7a152204 |
| رقم الأكسشن: | edsdoj.2778c5793cf44aa0a4c9ee0d7a152204 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 17560500 |
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| DOI: | 10.1186/s13104-022-06251-5 |