دورية أكاديمية
Downregulation of lncRNA TUG1 Affects Apoptosis and Insulin Secretion in Mouse Pancreatic β Cells
| العنوان: | Downregulation of lncRNA TUG1 Affects Apoptosis and Insulin Secretion in Mouse Pancreatic β Cells |
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| المؤلفون: | Dan-dan Yin, Er-bao Zhang, Liang-hui You, Ning Wang, Lin-tao Wang, Fei-yan Jin, Ya-nan Zhu, Li-hua Cao, Qing-xin Yuan, Wei De, Wei Tang |
| المصدر: | Cellular Physiology and Biochemistry, Vol 35, Iss 5, Pp 1892-1904 (2015) |
| بيانات النشر: | Cell Physiol Biochem Press GmbH & Co KG, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Physiology LCC:Biochemistry |
| مصطلحات موضوعية: | Apoptosis, Insulin synthesis, Insulin secretion, IncRNA TUG1, Physiology, QP1-981, Biochemistry, QD415-436 |
| الوصف: | Background: Increasing evidence indicates that long noncoding RNAs (IncRNAs) perform specific biological functions in diverse processes. Recent studies have reported that IncRNAs may be involved in β cell function. The aim of this study was to characterize the role of IncRNA TUG1 in mouse pancreatic β cell functioning both in vitro and in vivo. Methods: qRT-PCR analyses were performed to detect the expression of lncRNA TUG1 in different tissues. RNAi, MTT, TUNEL and Annexin V-FITC assays and western blot, GSIS, ELISA and immunochemistry analyses were performed to detect the effect of lncRNA TUG1 on cell apoptosis and insulin secretion in vitro and in vivo. Results: lncRNA TUG1 was highly expressed in pancreatic tissue compared with other organ tissues, and expression was dynamically regulated by glucose in Nit-1 cells. Knockdown of lncRNA TUG1 expression resulted in an increased apoptosis ratio and decreased insulin secretion in β cells both in vitro and in vivo . Immunochemistry analyses suggested decreased relative islet area after treatment with lncRNA TUG1 siRNA. Conclusion: Downregulation of lncRNA TUG1 expression affected apoptosis and insulin secretion in pancreatic β cells in vitro and in vivo. lncRNA TUG1 may represent a factor that regulates the function of pancreatic β cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1015-8987 1421-9778 |
| Relation: | http://www.karger.com/Article/FullText/373999; https://doaj.org/toc/1015-8987; https://doaj.org/toc/1421-9778 |
| DOI: | 10.1159/000373999 |
| URL الوصول: | https://doaj.org/article/27b9fd601c8c492d813b5c22485cd08d |
| رقم الأكسشن: | edsdoj.27b9fd601c8c492d813b5c22485cd08d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10158987 14219778 |
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| DOI: | 10.1159/000373999 |