دورية أكاديمية
Passivated Impedimetric Sensors for Immobilization-Free Pathogen Detection by Isothermal Amplification and Melt Curve Analysis
| العنوان: | Passivated Impedimetric Sensors for Immobilization-Free Pathogen Detection by Isothermal Amplification and Melt Curve Analysis |
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| المؤلفون: | Matthias Steinmaßl, Jamila Boudaden, Güven Edgü, Lena Julie Freund, Simon Meyer, Noa Mordehay, Melissa Soto, Hanns-Erik Endres, Jost Muth, Dirk Prüfer, Wilfried Lerch, Christoph Kutter |
| المصدر: | Biosensors, Vol 12, Iss 5, p 261 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biotechnology |
| مصطلحات موضوعية: | point-of-care, impedance, virus detection, LAMP, melt curve analysis, microheating, Biotechnology, TP248.13-248.65 |
| الوصف: | The ongoing SARS-CoV-2 pandemic demonstrates that the capacity of centralized clinical diagnosis laboratories represents a significant limiting factor in the global fight against the newly emerged virus. Scaling up these capacities also requires simple and robust methods for virus diagnosis to be easily driven by untrained personnel in a point-of-care (POC) environment. The use of impedance sensors reduces the complexity and costs of diagnostic instruments and increases automation of diagnosis processes. We present an impedance point-of-care system (IMP-POCS) that uses interdigitated electrodes surrounded by an integrated heating meander to monitor loop-mediated isothermal amplification (LAMP) and melt curve analysis (MCA) consecutively in a short time. MCA permits distinguishing false- from true-positive results and significantly raises the validity of pathogen detection. Conclusively, the herein-developed miniaturized total analysis system (µTAS) represents a powerful and promising tool for providing reliable, low-cost alternatives to standard clinical diagnosis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 12050261 2079-6374 |
| Relation: | https://www.mdpi.com/2079-6374/12/5/261; https://doaj.org/toc/2079-6374 |
| DOI: | 10.3390/bios12050261 |
| URL الوصول: | https://doaj.org/article/e27c45618a0b4395ba0cb84742b8c078 |
| رقم الأكسشن: | edsdoj.27c45618a0b4395ba0cb84742b8c078 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12050261 20796374 |
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| DOI: | 10.3390/bios12050261 |