دورية أكاديمية
A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells
| العنوان: | A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells |
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| المؤلفون: | Haishuang Lin, Qian Du, Qiang Li, Ou Wang, Zhanqi Wang, Neety Sahu, Christian Elowsky, Kan Liu, Chi Zhang, Soonkyu Chung, Bin Duan, Yuguo Lei |
| المصدر: | Stem Cell Reports, Vol 11, Iss 2, Pp 454-469 (2018) |
| بيانات النشر: | Elsevier, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | Summary: Endothelial cells (ECs) are of great value for cell therapy, tissue engineering, and drug discovery. Obtaining high-quantity and -quality ECs remains very challenging. Here, we report a method for the scalable manufacturing of ECs from human pluripotent stem cells (hPSCs). hPSCs are expanded and differentiated into ECs in a 3D thermoreversible PNIPAAm-PEG hydrogel. The hydrogel protects cells from hydrodynamic stresses in the culture vessel and prevents cells from excessive agglomeration, leading to high-culture efficiency including high-viability (>90%), high-purity (>80%), and high-volumetric yield (2.0 × 107 cells/mL). These ECs (i.e., 3D-ECs) had similar properties as ECs made using 2D culture systems (i.e., 2D-ECs). Genome-wide gene expression analysis showed that 3D-ECs had higher expression of genes related to vasculature development, extracellular matrix, and glycolysis, while 2D-ECs had higher expression of genes related to cell proliferation. : Endothelial cells (ECs) differentiated from human pluripotent stem cells (hPSCs) are needed in large numbers for regenerative medicine. In this article, Lei and colleagues describe a simple and innovative method for the scalable production of ECs from hPSCs. The method has potential to make ECs broadly available and affordable for various applications. Key words: human pluripotent stem cells, endothelial cells, 3D culture, thermoreversible hydrogel |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2213-6711 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2213671118303060; https://doaj.org/toc/2213-6711 |
| DOI: | 10.1016/j.stemcr.2018.07.001 |
| URL الوصول: | https://doaj.org/article/281209dcdad14c9090121d317c158c1f |
| رقم الأكسشن: | edsdoj.281209dcdad14c9090121d317c158c1f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22136711 |
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| DOI: | 10.1016/j.stemcr.2018.07.001 |