دورية أكاديمية
CircRNA PVT1 modulated cell migration and invasion through Epithelial-Mesenchymal Transition (EMT) mediation in gastric cancer through miR-423-5p/Smad3 pathway
| العنوان: | CircRNA PVT1 modulated cell migration and invasion through Epithelial-Mesenchymal Transition (EMT) mediation in gastric cancer through miR-423-5p/Smad3 pathway |
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| المؤلفون: | Huanqin Li, Shuai Xue, Xiaohong Zhang, Fan Li, Songhua Bei, Li Feng |
| المصدر: | Regenerative Therapy, Vol 21, Iss , Pp 25-33 (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) LCC:Cytology |
| مصطلحات موضوعية: | Gastric cancer, CircPVT1, miR-423-5p, Smad3 pathway, Medicine (General), R5-920, Cytology, QH573-671 |
| الوصف: | Background: Gastric cancer (GC) progression is related with gene regulations. Objectives: This study explored underlying regulatory axis of circRNA PVT1 (circPVT1) in GC. Methods: GC cell lines were detected for circPVT1 expression with the normal mucous epithelial cell GES-1 as control. After regulation of circPVT1, miR-423-5p and SMAD3 expression through transfection, CCK8 evaluated the cell viability, Transwell measured the migratory and invasive capability of cells. Luciferase verified the paired bindings between miR-423-5p and CircPVT1 or SMAD3. The functions of CircPVT1/miR-423-5p/SMAD3 were evaluated using RT-PCR, CCK8, Transwell assays. Western blot analyzed EMT-related proteins and phosphorylation of Smad3 in GC cells. Immunofluorescence method was used to evaluate the EMT-related proteins as well. Results: CircPVT1 displayed higher expression in GC cells and knockdown led to decrease in cell growth, invasion and migration. CircPVT1 was targeted by miR-423-5p as a ceRNA of SMAD3. miR-423-5p upregulation suppressed both cicRNA PVT1 and SMAD3 in GC cells. Decrease in SMAD3 expression suppressed CircPVT1 by releasing miR-423-5p in cells, inhibiting cell growth, invasion and migration and suppressing the EMT process. Conclusion: CircPVT1 modulated cell growth, invasion and migration through EMT mediation in gastric cancer through miR-423-5p/Smad3 pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2352-3204 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2352320422000128; https://doaj.org/toc/2352-3204 |
| DOI: | 10.1016/j.reth.2022.02.003 |
| URL الوصول: | https://doaj.org/article/28f1c1b1792d42639bb05c37375bb31b |
| رقم الأكسشن: | edsdoj.28f1c1b1792d42639bb05c37375bb31b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23523204 |
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| DOI: | 10.1016/j.reth.2022.02.003 |