دورية أكاديمية
Transcriptomic and proteomic responses to very low CO2 suggest multiple carbon concentrating mechanisms in Nannochloropsis oceanica
| العنوان: | Transcriptomic and proteomic responses to very low CO2 suggest multiple carbon concentrating mechanisms in Nannochloropsis oceanica |
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| المؤلفون: | Li Wei, Mohamed El Hajjami, Chen Shen, Wuxin You, Yandu Lu, Jing Li, Xiaoyan Jing, Qiang Hu, Wenxu Zhou, Ansgar Poetsch, Jian Xu |
| المصدر: | Biotechnology for Biofuels, Vol 12, Iss 1, Pp 1-21 (2019) |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Fuel LCC:Biotechnology |
| مصطلحات موضوعية: | Carbon fixation, Carbon concentrating mechanism (CCM), C4-like cycle, Industrial oleaginous microalgae, Nannochloropsis oceanica, Fuel, TP315-360, Biotechnology, TP248.13-248.65 |
| الوصف: | Abstract Background In industrial oleaginous microalgae such as Nannochloropsis spp., the key components of the carbon concentration mechanism (CCM) machineries are poorly defined, and how they are mobilized to facilitate cellular utilization of inorganic carbon remains elusive. Results For Nannochloropsis oceanica, to unravel genes specifically induced by CO2 depletion which are thus potentially underpinning its CCMs, transcriptome, proteome and metabolome profiles were tracked over 0 h, 3 h, 6 h, 12 h and 24 h during cellular response from high CO2 level (HC; 50,000 ppm) to very low CO2 (VLC; 100 ppm). The activity of a biophysical CCM is evidenced based on induction of transcripts encoding a bicarbonate transporter and two carbonic anhydrases under VLC. Moreover, the presence of a potential biochemical CCM is supported by the upregulation of a number of key C4-like pathway enzymes in both protein abundance and enzymatic activity under VLC, consistent with a mitochondria-implicated C4-based CCM. Furthermore, a basal CCM underpinned by VLC-induced upregulation of photorespiration and downregulation of ornithine–citrulline shuttle and the ornithine urea cycles is likely present, which may be responsible for efficient recycling of mitochondrial CO2 for chloroplastic carbon fixation. Conclusions Nannochloropsis oceanica appears to mobilize a comprehensive set of CCMs in response to very low CO2. Its genes induced by the stress are quite distinct from those of Chlamydomonas reinhardtii and Phaeodactylum tricornutum, suggesting tightly regulated yet rather unique CCMs. These findings can serve the first step toward rational engineering of the CCMs for enhanced carbon fixation and biomass productivity in industrial microalgae. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1754-6834 |
| Relation: | http://link.springer.com/article/10.1186/s13068-019-1506-8; https://doaj.org/toc/1754-6834 |
| DOI: | 10.1186/s13068-019-1506-8 |
| URL الوصول: | https://doaj.org/article/29061a577714423b86abd9fb1cd39d5f |
| رقم الأكسشن: | edsdoj.29061a577714423b86abd9fb1cd39d5f |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 17546834 |
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| DOI: | 10.1186/s13068-019-1506-8 |