دورية أكاديمية

Superior Potential of CD34-Positive Cells Compared to Total Mononuclear Cells for Healing of Nonunion following Bone Fracture

التفاصيل البيبلوغرافية
العنوان: Superior Potential of CD34-Positive Cells Compared to Total Mononuclear Cells for Healing of Nonunion following Bone Fracture
المؤلفون: Tomoaki Fukui, Yutaka Mifune, Tomoyuki Matsumoto, Taro Shoji, Yohei Kawakami, Atsuhiko Kawamoto, Masaaki Ii, Hiroshi Akimaru, Tomoya Kuroda, Miki Horii, Ayumi Yokoyama, Cantas Alev, Ryosuke Kuroda, Masahiro Kurosaka, Takayuki Asahara M.D., Ph.D.
المصدر: Cell Transplantation, Vol 24 (2015)
بيانات النشر: SAGE Publishing, 2015.
سنة النشر: 2015
المجموعة: LCC:Medicine
مصطلحات موضوعية: Medicine
الوصف: We recently demonstrated that the local transplantation of human peripheral blood (PB) CD34 + cells, an endothelial/hematopoietic progenitor cell-rich population, contributes to fracture repair via vasculogenesis/angiogenesis and osteogenesis. Human PB mononuclear cells (MNCs) are also considered a potential cell fraction for neovascularization. We have previously shown the feasibility of human PB MNCs to enhance fracture healing. However, there is no report directly comparing the efficacy for fracture repair between CD34 + cells and MNCs. In addition, an unhealing fracture model, which does not accurately resemble a clinical setting, was used in our previous studies. To overcome these issues, we compared the capacity of human granulocyte colony-stimulating factor-mobilized PB (GM-PB) CD34 + cells and human GM-PB MNCs in a nonunion model, which more closely resembles a clinical setting. First, the effect of local transplantation of 1 × 10 5 GM-PB CD34 + cells (CD34 + group), 1 × 10 7 GM-PB MNCs (containing approximately 1 × 10 5 GM-PB CD34 + cells) (MNC group), and phosphate-buffered saline (PBS) (PBS group) on nonunion healing was compared. Similar augmentation of blood flow recovery at perinonunion sites was observed in the CD34 + and MNC groups. Meanwhile, a superior effect on nonunion repair was revealed by radiological, histological, and functional assessment in the CD34 + group compared with the other groups. Moreover, through in vivo and in vitro experiments, excessive inflammation induced by GM-PB MNCs was confirmed and believed to be one of the mechanisms underlying this potency difference. These results strongly suggest that local transplantation of GM-PB CD34 + cells is a practical and effective strategy for treatment of nonunion after fracture.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 0963-6897
1555-3892
Relation: https://doaj.org/toc/0963-6897; https://doaj.org/toc/1555-3892
DOI: 10.3727/096368914X681586
URL الوصول: https://doaj.org/article/2933f687f1c84ad6b01e267cd92be207
رقم الأكسشن: edsdoj.2933f687f1c84ad6b01e267cd92be207
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:09636897
15553892
DOI:10.3727/096368914X681586