دورية أكاديمية
Differential expression and localization of tight junction proteins in the goat epididymis
| العنوان: | Differential expression and localization of tight junction proteins in the goat epididymis |
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| المؤلفون: | Sung Woo Kim, Yu-Da Jeong, Ga-Yeong Lee, Jinwook Lee, Jae-Yeung Lee, Chan-Lan Kim, Yeoung-Gyu Ko, Sung-Soo Lee, Bongki Kim |
| المصدر: | Journal of Animal Science and Technology, Vol 64, Iss 3, Pp 500-514 (2022) |
| بيانات النشر: | Korean Society of Animal Sciences and Technology, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Animal culture |
| مصطلحات موضوعية: | blood-epididymis barrier, tight junctions, zonula occludens, claudin, occludin, Animal culture, SF1-1100 |
| الوصف: | The blood-epididymis barrier (BEB) forms a unique microenvironment that is crucial for the maturation, protection, transport, and storage of spermatozoa in the epididymis. To characterize the function of tight junctions (TJs), which are constitutive components of the BEB, we determined the expression and localization of TJ proteins such as zonula occludens (ZO)-1, 2, and 3, occludin, and claudin3 (Cldn3) during postnatal development in the goat epididymis. To assess the expression patterns of TJ proteins in immature (3 months of age) and mature (14 months of age) goat epididymides, two different experimental methods were used including immunofluorescence labeling and western blotting. We show that, ZO-1, 2, and 3, and occludin, were strictly expressed and localized to the TJs of the goat epididymis, whereas Cldn3 was present in basolateral membranes as well as TJs. All TJ proteins examined were more highly expressed in the immature epididymis compared to levels in mature tissue. In conclusion, our study indicates that at least five TJ proteins, namely ZO-1, ZO-2, ZO-3, occludin, and Cldn3, are present in TJs, and the expression strength and pattern of TJ proteins tend to be age dependent in the goat epididymis. Together, these data suggest that the distinct expression patterns of TJ proteins are essential for regulating components of the luminal contents in the epididymal epithelium and for forming adequate luminal conditions that are necessary for the maturation, protection, transport, and storage of spermatozoa in the goat epididymis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2672-0191 2055-0391 |
| Relation: | http://www.ejast.org/archive/view_article?pid=jast-64-3-500; https://doaj.org/toc/2672-0191; https://doaj.org/toc/2055-0391 |
| DOI: | 10.5187/jast.2022.e13 |
| URL الوصول: | https://doaj.org/article/d294e685c9e54138883cdc601953df3a |
| رقم الأكسشن: | edsdoj.294e685c9e54138883cdc601953df3a |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 26720191 20550391 |
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| DOI: | 10.5187/jast.2022.e13 |