دورية أكاديمية

Keratins and plakin family cytolinker proteins control the length of epithelial microridge protrusions

التفاصيل البيبلوغرافية
العنوان: Keratins and plakin family cytolinker proteins control the length of epithelial microridge protrusions
المؤلفون: Yasuko Inaba, Vasudha Chauhan, Aaron Paul van Loon, Lamia Saiyara Choudhury, Alvaro Sagasti
المصدر: eLife, Vol 9 (2020)
بيانات النشر: eLife Sciences Publications Ltd, 2020.
سنة النشر: 2020
المجموعة: LCC:Medicine
LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: keratin, actin, Plakin, morphogenesis, protrusion, microridge, Medicine, Science, Biology (General), QH301-705.5
الوصف: Actin filaments and microtubules create diverse cellular protrusions, but intermediate filaments, the strongest and most stable cytoskeletal elements, are not known to directly participate in the formation of protrusions. Here we show that keratin intermediate filaments directly regulate the morphogenesis of microridges, elongated protrusions arranged in elaborate maze-like patterns on the surface of mucosal epithelial cells. We found that microridges on zebrafish skin cells contained both actin and keratin filaments. Keratin filaments stabilized microridges, and overexpressing keratins lengthened them. Envoplakin and periplakin, plakin family cytolinkers that bind F-actin and keratins, localized to microridges, and were required for their morphogenesis. Strikingly, plakin protein levels directly dictate microridge length. An actin-binding domain of periplakin was required to initiate microridge morphogenesis, whereas periplakin-keratin binding was required to elongate microridges. These findings separate microridge morphogenesis into distinct steps, expand our understanding of intermediate filament functions, and identify microridges as protrusions that integrate actin and intermediate filaments.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2050-084X
Relation: https://elifesciences.org/articles/58149; https://doaj.org/toc/2050-084X
DOI: 10.7554/eLife.58149
URL الوصول: https://doaj.org/article/29b0840fd46346ff9c29d04f791f0492
رقم الأكسشن: edsdoj.29b0840fd46346ff9c29d04f791f0492
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2050084X
DOI:10.7554/eLife.58149