دورية أكاديمية
Methyladenosine modification (mA) of circRNA-ZNF638 contributes to the induced activation of SHF stem cells through miR-361-5p/Wnt5a axis in cashmere goats
| العنوان: | Methyladenosine modification (mA) of circRNA-ZNF638 contributes to the induced activation of SHF stem cells through miR-361-5p/Wnt5a axis in cashmere goats |
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| المؤلفون: | Ronghuan Yin, Ronglan Yin, Man Bai, Yixing Fan, Zeying Wang, Yubo Zhu, Qi Zhang, Taiyu Hui, Jincheng Shen, Siyu Feng, Wenlin Bai |
| المصدر: | Animal Bioscience, Vol 36, Iss 4, Pp 555-569 (2023) |
| بيانات النشر: | Asian-Australasian Association of Animal Production Societies, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Zoology |
| مصطلحات موضوعية: | cashmere goats, circrna-znf638, mir-361-5p, -methyladenosine, shf stem cells, wnt5a, Zoology, QL1-991 |
| الوصف: | Objective The objective of this study was to investigate the effects of N6-Methyladenosine modification-circRNA-zinc finger protein 638 (m6A-circRNA-ZNF638) on the induced activation of secondary hair follicle (SHF) stem cells with its potential mechanisms in cashmere goats. Methods The m6A modification of ZNF638 was analyzed using methylation immunoprecipitation with real-time quantitative polymerase chain reaction technique in SHF stem cells. The effects of circRNA-ZNF638 on the induced activation of SHF stem cells in m6A dependence were evaluated through the overexpression of circRNA-ZNF638/its m6A-deficient mutants in circRNA-ZNF638 knockdown SHF stem cells. The competitive binding of miR-361-5p to circRNA-ZNF638/Wnt5a 3′-untranslated region was analyzed through Dual-luciferase reporter assay. Results The m6A-circRNA-ZNF638 had significantly higher transcription at anagen SHF bulge of cashmere goats compared with that at telogen, as well as it positively regulated the induced activation of SHF-stem cells in cashmere goats. Mechanismly, m6A-circRNA-ZNF638 sponged miR-361-5p to heighten the transcriptional expression of Wnt5a gene in SHF-stem cells. We further demonstrated that the internal m6A modification within circRNA-ZNF638 is required for mediating the miR-361-5p/Wnt5a pathway to regulate the induced activation of SHF stem cells through an introducing of m6A-deficient mutant of circRNA-ZNF638. Conclusion The circRNA-ZNF638 contributes the proper induced activation of SHF-stem cells in cashmere goats in m6A-dependent manner through miR-361-5p/Wnt5a axis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2765-0189 2765-0235 |
| Relation: | http://www.animbiosci.org/upload/pdf/ab-22-0211.pdf; https://doaj.org/toc/2765-0189; https://doaj.org/toc/2765-0235 |
| DOI: | 10.5713/ab.22.0211 |
| URL الوصول: | https://doaj.org/article/d2a62bf50f9a4eef8a831c340229909f |
| رقم الأكسشن: | edsdoj.2a62bf50f9a4eef8a831c340229909f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 27650189 27650235 |
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| DOI: | 10.5713/ab.22.0211 |