دورية أكاديمية
Development of a single nucleotide polymorphism–based strain-identified method for Streptococcus thermophilus CICC 6038 and Lactobacillus delbrueckii ssp. bulgaricus CICC 6047 using pan-genomics analysis
| العنوان: | Development of a single nucleotide polymorphism–based strain-identified method for Streptococcus thermophilus CICC 6038 and Lactobacillus delbrueckii ssp. bulgaricus CICC 6047 using pan-genomics analysis |
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| المؤلفون: | Zhiquan Song, Yuanyuan Ge, Xuejian Yu, Rui Liu, Chong Liu, Kun Cheng, Lizheng Guo, Su Yao |
| المصدر: | Journal of Dairy Science, Vol 107, Iss 7, Pp 4248-4258 (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Dairy processing. Dairy products |
| مصطلحات موضوعية: | probiotics, pan-genome, single nucleotide polymorphisms, strain-specific identification, Dairy processing. Dairy products, SF250.5-275, Dairying, SF221-250 |
| الوصف: | ABSTRACT: The health benefits conferred by probiotics is specific to individual probiotic strains, highlighting the importance of identifying specific strains for research and production purposes. Streptococcus thermophilus CICC 6038 and Lactobacillus delbrueckii ssp. bulgaricus CICC 6047 are exceedingly valuable for commercial use with an excellent mixed-culture fermentation. To differentiate these 2 strains from other S. thermophilus and L. delbrueckii ssp. bulgaricus, a specific, sensitive, accurate, rapid, convenient, and cost-effective method is required. In this study, we conducted a pan-genome analysis of S. thermophilus and L. delbrueckii ssp. bulgaricus to identify species-specific core genes, along with strain-specific SNPs. These genes were used to develop suitable PCR primers, and the conformity of sequence length and unique SNPs was confirmed by sequencing for qualitative identification at the strain level. The results demonstrated that SNPs analysis of PCR products derived from these primers could distinguish CICC 6038 and CICC 6047 accurately and reproducibly from the other strains of S. thermophilus and L. delbrueckii ssp. bulgaricus, respectively. The strain-specific PCR method based on SNPs herein is universally applicable for probiotics identification. It offers valuable insights into identifying probiotics at the strain level that is fit-for-purpose in quality control and compliance assessment of commercial dairy products. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0022-0302 2023-2365 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0022030224000146; https://doaj.org/toc/0022-0302 |
| DOI: | 10.3168/jds.2023-23655 |
| URL الوصول: | https://doaj.org/article/2b3ae6d449fa4825879b7e39c1785eec |
| رقم الأكسشن: | edsdoj.2b3ae6d449fa4825879b7e39c1785eec |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 00220302 20232365 |
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| DOI: | 10.3168/jds.2023-23655 |