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Dynamics of CD44+ bovine nucleus pulposus cells with inflammation

التفاصيل البيبلوغرافية
العنوان: Dynamics of CD44+ bovine nucleus pulposus cells with inflammation
المؤلفون: J. R. Ferreira, J. Caldeira, M. Sousa, M. A. Barbosa, M. Lamghari, G. Almeida-Porada, R. M. Gonçalves
المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-15 (2024)
بيانات النشر: Nature Portfolio, 2024.
سنة النشر: 2024
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Inflammation, Intervertebral disc, CD44, Proteomics, IL-1β, Medicine, Science
الوصف: Abstract Intervertebral Disc (IVD) degeneration has been associated with a chronic inflammatory response, but knowledge on the contribution of distinct IVD cells, namely CD44, to the progression of IVD degeneration remains elusive. Here, bovine nucleus pulposus (NP) CD44 cells were sorted and compared by gene expression and proteomics with the negative counterpart. NP cells were then stimulated with IL-1b (10 ng/ml) and dynamics of CD44 gene and protein expression was analyzed upon pro-inflammatory treatment. The results emphasize that CD44 has a multidimensional functional role in IVD metabolism, ECM synthesis and production of neuropermissive factors. CD44 widespread expression in NP was partially associated with CD14 and CD45, resulting in the identification of distinct cell subsets. In conclusion, this study points out CD44 and CD44-based cell subsets as relevant targets in the modulation of the IVD pro-inflammatory/degenerative cascade.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-2322
Relation: https://doaj.org/toc/2045-2322
DOI: 10.1038/s41598-024-59504-7
URL الوصول: https://doaj.org/article/c2ca574b9e69447fa96468dc375be62c
رقم الأكسشن: edsdoj.2ca574b9e69447fa96468dc375be62c
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20452322
DOI:10.1038/s41598-024-59504-7