دورية أكاديمية
أعرض في EDS

Pathogen Box screening for hit identification against Mycobacterium abscessus.

التفاصيل البيبلوغرافية
العنوان: Pathogen Box screening for hit identification against Mycobacterium abscessus.
المؤلفون: Jinsun Jeong, Guehye Kim, Cheol Moon, Hyun Jung Kim, Tae Ho Kim, Jichan Jang
المصدر: PLoS ONE, Vol 13, Iss 4, p e0195595 (2018)
بيانات النشر: Public Library of Science (PLoS), 2018.
سنة النشر: 2018
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Mycobacterium abscessus is a rapidly growing life-threatening mycobacterium with multiple drug-resistance mechanisms. However, there is no official regimen for M. abscessus therapy. In this study, we screened the Pathogen Box, which contains 400 drug-like molecules active against neglected diseases, to identify active molecules targeting Mycobacterium abscessus using resazurin live/dead assays. In this screening assay, the Z-factor was 0.7, as an indicator of the statistical confidence of the assay. A cut-off of 80% growth inhibition in the screening resulted in the identification of four different compounds at a single concentration (20 μM). Dose-response curves identified three different hit candidates, i.e., MMV688508, MMV688844, and MMV688845, which generated good inhibitory curves. All hit candidates were expected to have different molecular targets. Among them, MMV688844 showed the best minimum inhibitory concentration value for not only wild-type M. abscessus but also for nine different R and S morphotype clinical isolates. Thus, we found that MMV688844, identified from the Pathogen Box screen, may be a promising candidate in the M. abscessus drug discovery pipeline.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC5919404?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0195595
URL الوصول: https://doaj.org/article/d2d6b4921b7642be81e0ab557b206e5c
رقم الأكسشن: edsdoj.2d6b4921b7642be81e0ab557b206e5c
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0195595