دورية أكاديمية
TLR7 Ligation Inhibits TLR8 Responsiveness in IL-27-Primed Human THP-1 Monocytes and Macrophages
| العنوان: | TLR7 Ligation Inhibits TLR8 Responsiveness in IL-27-Primed Human THP-1 Monocytes and Macrophages |
|---|---|
| المؤلفون: | Natalya Odoardi, Olena Kourko, Carlene Petes, Sameh Basta, Katrina Gee |
| المصدر: | Journal of Innate Immunity, Pp 1-14 (2021) |
| بيانات النشر: | Karger Publishers, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine LCC:Internal medicine |
| مصطلحات موضوعية: | interleukin-27, toll-like receptor 7, toll-like receptor 8, toll-like receptor, interferon, monocytes, macrophages, inflammation, Medicine, Internal medicine, RC31-1245 |
| الوصف: | Regulation of proinflammatory cytokine expression is critical in the face of single-stranded RNA (ssRNA) virus infections. Many viruses, including coronavirus and influenza virus, wreak havoc on the control of cytokine expression, leading to the formation of detrimental cytokine storms. Understanding the regulation and interplay between inflammatory cytokines is critical to the identification of targets involved in controlling the induction of cytokine expression. In this study, we focused on how the antiviral cytokine interleukin-27 (IL-27) regulates signal transduction downstream of Toll-like receptor 7 (TLR7) and TLR8 ligation, which recognize endosomal single-stranded RNA. Given that IL-27 alters bacterial-sensing TLR expression on myeloid cells and can inhibit replication of single-stranded RNA viruses, we investigated whether IL-27 affects expression and function of TLR7 and TLR8. Analysis of IL-27-treated THP-1 monocytic cells and THP-1-derived macrophages revealed changes in mRNA and protein expression of TLR7 and TLR8. Although treatment with IL-27 enhanced TLR7 expression, only TLR8-mediated cytokine secretion was amplified. Furthermore, we demonstrated that imiquimod, a TLR7 agonist, inhibited cytokine and chemokine production induced by a TLR8 agonist, TL8-506. Delineating the immunomodulatory role of IL-27 on TLR7 and TLR8 responses provides insight into how myeloid cell TLR-mediated responses are regulated during virus infection. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1662-811X 1662-8128 |
| Relation: | https://www.karger.com/Article/FullText/515738; https://doaj.org/toc/1662-811X; https://doaj.org/toc/1662-8128 |
| DOI: | 10.1159/000515738 |
| URL الوصول: | https://doaj.org/article/2e088aacb02248b18bcf860afc08d504 |
| رقم الأكسشن: | edsdoj.2e088aacb02248b18bcf860afc08d504 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1662811X 16628128 |
|---|---|
| DOI: | 10.1159/000515738 |