دورية أكاديمية
Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin
| العنوان: | Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin |
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| المؤلفون: | N. Bracesco, V. Sosa, L. Blanc, V. Contreras, E.C. Candreva, V.A. Salvo, S. Hocart, B. Mechoso, E. Nunes |
| المصدر: | Brazilian Journal of Medical and Biological Research, Vol 51, Iss 9 (2018) |
| بيانات النشر: | Associação Brasileira de Divulgação Científica, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | Radiation damage, Ilex paraguariensis infusion, Rutin, Radioprotection, Antimutagenic effects, Saccharomyces cerevisiae, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | DNA repair pathways, cell cycle checkpoints, and redox protection systems are essential factors for securing genomic stability. The aim of the present study was to analyze the effect of Ilex paraguariensis (Ip) infusion and one of its polyphenolic components rutin on cellular and molecular damage induced by ionizing radiation. Ip is a beverage drank by most inhabitants of Argentina, Paraguay, Southern Brazil, and Uruguay. The yeast Saccharomyces cerevisiae (SC7Klys 2-3) was used as the eukaryotic model. Exponentially growing cells were exposed to gamma rays (γ) in the presence or absence of Ip or rutin. The concentrations used simulated those found in the habitual infusion. Surviving fractions, mutation frequency, and DNA double-strand breaks (DSB) were determined after treatments. A significant increase in surviving fractions after gamma irradiation was observed following combined exposure to γ+R, or γ+Ip. Upon these concomitant treatments, mutation and DSB frequency decreased significantly. In the mutant strain deficient in MEC1, a significant increase in γ sensitivity and a low effect of rutin on γ-induced chromosomal fragmentation was observed. Results were interpreted in the framework of a model of interaction between radiation-induced free radicals, DNA repair pathways, and checkpoint controls, where the DNA damage that induced activation of MEC1 nodal point of the network could be modulated by Ip components including rutin. Furthermore, ionizing radiation-induced redox cascades can be interrupted by rutin potential and other protectors contained in Ip. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1414-431X 1414-431x |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900606&lng=en&tlng=en; https://doaj.org/toc/1414-431X |
| DOI: | 10.1590/1414-431x20187404 |
| URL الوصول: | https://doaj.org/article/2e1164aa4ab44320beaee446481247c7 |
| رقم الأكسشن: | edsdoj.2e1164aa4ab44320beaee446481247c7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1414431X 1414431x |
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| DOI: | 10.1590/1414-431x20187404 |