دورية أكاديمية
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EphA receptors regulate prostate cancer cell dissemination through Vav2–RhoA mediated cell–cell repulsion

التفاصيل البيبلوغرافية
العنوان: EphA receptors regulate prostate cancer cell dissemination through Vav2–RhoA mediated cell–cell repulsion
المؤلفون: Jennifer Batson, Lucy Maccarthy-Morrogh, Amy Archer, Helen Tanton, Catherine D. Nobes
المصدر: Biology Open, Vol 3, Iss 6, Pp 453-462 (2014)
بيانات النشر: The Company of Biologists, 2014.
سنة النشر: 2014
المجموعة: LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: Eph receptors, Contact inhibition of locomotion, Cell migration, Prostate cancer, Rho GTPases, Vav2, Science, Biology (General), QH301-705.5
الوصف: Metastatic prostate cancer cells display EphB receptor-mediated attraction when they contact stromal fibroblasts but EphA-driven repulsion when they contact one another. The impact of these ‘social’ interactions between cells during cancer cell invasion and the signalling mechanisms downstream of Eph receptors are unclear. Here we show that EphA receptors regulate prostate cancer cell dissemination in a 2D dispersal assay and in a 3D cancer cell spheroid assay. We show that EphA receptors signal via the exchange factor Vav2 to activate RhoA and that both Vav2 and RhoA are required for prostate cancer cell–cell repulsion. Furthermore, we find that in EphA2/EphA4, Vav2 or RhoA siRNA-treated cells, contact repulsion can be restored by partial microtubule destabilisation. We propose that EphA–Vav2–RhoA-mediated repulsion between contacting cancer cells at the tumour edge could enhance their local invasion away from the primary tumour.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2046-6390
Relation: http://bio.biologists.org/content/3/6/453; https://doaj.org/toc/2046-6390
DOI: 10.1242/bio.20146601
URL الوصول: https://doaj.org/article/2eb1e46e80cc4047a6cef51236b2a410
رقم الأكسشن: edsdoj.2eb1e46e80cc4047a6cef51236b2a410
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20466390
DOI:10.1242/bio.20146601