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أعرض في EDS

Parasite Burden of Trypanosoma cruzi in Whole Blood and Buffy Coat Determined by Real-Time PCR in Individuals with Chronic Chagas Disease

التفاصيل البيبلوغرافية
العنوان: Parasite Burden of Trypanosoma cruzi in Whole Blood and Buffy Coat Determined by Real-Time PCR in Individuals with Chronic Chagas Disease
المؤلفون: Daniela Liempi, Inés Zulantay, Nelson M. Varela, Mauricio Canals, Andrés Guevara, Nicolás Poulsen, Werner Apt
المصدر: Microorganisms, Vol 12, Iss 2, p 249 (2024)
بيانات النشر: MDPI AG, 2024.
سنة النشر: 2024
المجموعة: LCC:Biology (General)
مصطلحات موضوعية: chronic Chagas disease, buffy coat, guanidine, parasitemia, real-time PCR, Biology (General), QH301-705.5
الوصف: The objective of this study was to compare, by qPCR, the circulating blood parasite load of Trypanosoma cruzi in the buffy coat, and in whole blood mixed with boiled and unboiled guanidine hydrochloride-EDTA buffer, of individuals with chronic ChD. The concentration and purity of DNA were evaluated in a Nanodrop Denovix DS-11FX Series Spectrophotometer (DeNovix Inc., Wilmington, NC, USA). The parasite load was determined with the Taqman® qPCR system using a Stratagene Mx3000P thermocycler (Agilent Technologies, Santa Clara, CA, USA) with Cruzi 1 and Cruzi 2 satellite primers. Student’s t-test with Bonferroni correction, Chi-squared (χ2) tests and Spearman’s correlation coefficient were applied. The concentration and purity of DNA were higher in the buffy coat. Parasite DNA was detected and quantifiable in the three types of samples in seven patients, without statistically significant differences in the parasite load obtained. Higher correlations were found between the total DNA concentrations and the parasite loads obtained in the samples of the buffy coat.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 12020249
2076-2607
Relation: https://www.mdpi.com/2076-2607/12/2/249; https://doaj.org/toc/2076-2607
DOI: 10.3390/microorganisms12020249
URL الوصول: https://doaj.org/article/2ed1a9be25d9478c82ecb2427808ed51
رقم الأكسشن: edsdoj.2ed1a9be25d9478c82ecb2427808ed51
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:12020249
20762607
DOI:10.3390/microorganisms12020249